Evidence map›Paper›PMID 39407217›Full record

ArticleBiotechnology for biofuels and bioproducts2024

Engineered reduction of S-adenosylmethionine alters lignin in sorghum.

Yang Tian, Yu Gao, Halbay Turumtay, Emine Akyuz Turumtay, Yen Ning Chai, Hemant Choudhary, Joon-Hyun Park, Chuan-Yin Wu, Christopher M De Ben, Jutta Dalton and 16 more

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Article in Biotechnology for biofuels and bioproducts, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Yang TianJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Yu GaoJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Halbay TurumtayJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Emine Akyuz TurumtayJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Yen Ning ChaiJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Hemant ChoudharyJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Joon-Hyun ParkForage Genetics International, West Salem, WI, 54669, USA.
Chuan-Yin WuForage Genetics International, West Salem, WI, 54669, USA.
Christopher M De BenDepartment of Plant Sciences, University of California-Davis, Davis, CA, 95616, USA.
Jutta DaltonJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Katherine B LouieEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 978R4468, Berkeley, CA, 94720, USA.
Thomas HarwoodEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 978R4468, Berkeley, CA, 94720, USA.
Dylan ChinJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Khanh M VuuJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Benjamin P BowenEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 978R4468, Berkeley, CA, 94720, USA.
Patrick M ShihJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Edward E K BaidooJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Trent R NorthenJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Blake A SimmonsJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Robert HutmacherJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Jackie AtimJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Daniel H PutnamJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Corinne D ScownJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Jenny C MortimerJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Henrik V SchellerJoint BioEnergy Institute, Emeryville, CA, 94608, USA.
Aymerick EudesJoint BioEnergy Institute, Emeryville, CA, 94608, USA. ageudes@lbl.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLignin is an aromatic polymer deposited in secondary cell walls of higher plants to provide strength, rigidity, and hydrophobicity to vascular tissues. Due to its interconnections with cell wall polysaccharides, lignin plays important roles during plant growth and defense, but also has a negative impact on industrial processes aimed at obtaining monosaccharides from plant biomass. Engineering lignin offers a solution to this issue. For example, previous work showed that heterologous expression of a coliphage S-adenosylmethionine hydrolase (AdoMetase) was an effective approach to reduce lignin in the model plant Arabidopsis. The efficacy of this engineering strategy remains to be evaluated in bioenergy crops.

resultsWe studied the impact of expressing AdoMetase on lignin synthesis in sorghum (Sorghum bicolor L. Moench). Lignin content, monomer composition, and size, as well as biomass saccharification efficiency were determined in transgenic sorghum lines. The transcriptome and metabolome were analyzed in stems at three developmental stages. Plant growth and biomass composition was further evaluated under field conditions. Results evidenced that lignin was reduced by 18% in the best transgenic line, presumably due to reduced activity of the S-adenosylmethionine-dependent O-methyltransferases involved in lignin synthesis. The modified sorghum features altered lignin monomer composition and increased lignin molecular weights. The degree of methylation of glucuronic acid on xylan was reduced. These changes enabled a ~20% increase in glucose yield after biomass pretreatment and saccharification compared to wild type. RNA-seq and untargeted metabolomic analyses evidenced some pleiotropic effects associated with AdoMetase expression. The transgenic sorghum showed developmental delay and reduced biomass yields at harvest, especially under field growing conditions.

conclusionsThe expression of AdoMetase represents an effective lignin engineering approach in sorghum. However, considering that this strategy potentially impacts multiple S-adenosylmethionine-dependent methyltransferases, adequate promoters for fine-tuning AdoMetase expression will be needed to mitigate yield penalty.

Indexed as

Bioenergy cropCell wallMonolignolsO-methyltransferasesSaccharification

Identifiers

PMID39407217
PMCPMC11481400

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.