Evidence map›Paper›PMID 39402219›Full record

ArticleRice (New York, N.Y.)2024

A Cyclin Gene OsCYCB1;5 Regulates Seed Callus Induction in Rice Revealed by Genome Wide Association Study.

Wenjing Song, Jian Zhang, Wenyu Lu, Siyi Liang, Hairong Cai, Yuanyuan Guo, Shiyi Chen, Jiafeng Wang, Tao Guo, Hong Liu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rice (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wenjing Song *National Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Jian Zhang *National Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Wenyu LuNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Siyi LiangNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Hairong CaiNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Yuanyuan GuoNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Shiyi ChenNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Jiafeng WangNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Tao GuoNational Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Hong LiuCollege of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China. laoliuhongscau@163.com.
Dehua RaoCollege of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China. raodehua@scau.edu.cn.

Funding

Analysis of agronomic and economic traits of superior rice varieties 2020KJ382-05DUS testing and database construction for rice, corn and other varieties h20231012
6 · The paper itself

Abstract

Plant tissue culture is extensively employed in plant functional genomics research and crop genetic improvement breeding. The callus induction ability is critical for utilizing Agrobacterium-mediated genetic transformation. In this study, we conducted a genome-wide association study (GWAS) utilizing 368 rice accessions to identify traits associated with callus induction rate (CIR), resulting in the identification of a total of 104 significant SNP loci. Integrated with gene function annotation and transcriptome analysis, 13 high-confidence candidate genes involved in auxin-related, CYC cyclins, and histone H3K9-specific methyltransferase were identified in significant loci. Furthermore, we also verified a candidate gene, Os05g0493500 (OsCycB1;5), and employed the CRISPR/Cas9 system to generate OsCycB1;5 knockout mutants in rice (Oryza sativa L.). The OscycB1;5 mutant displays significantly reduced callus induction and proliferation capacity, this result indicating OsCycB1;5 is required for the callus formation in rice. Overall, this study provides several reliable loci and high-confidence candidate genes that may significantly affect callus formation in rice. This information will offer valuable insights into the mechanisms underlying callus formation not only in rice but also in other plants.

Indexed as

5Callus induction rateCandidate geneGWASOsCycB1Rice

Identifiers

PMID39402219
PMCPMC11473481

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.