Evidence map›Paper›PMID 39401898›Full record

ReviewProceedings of the Japan Academy. Series B, Physical and biological sciences2024

Crystals of taka-amylase A, a cornerstone of protein chemistry in Japan.

Saburo Aimoto, Naoto Minamino, Takeshi Ishimizu, Masami Kusunoki

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Review in Proceedings of the Japan Academy. Series B, Physical and biological sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

4 authors.

Saburo AimotoProfessor Emeritus, Osaka University, Suita, Osaka, Japan.ORCID http://orcid.org/0009-0001-0201-476X
Naoto MinaminoNational Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.ORCID http://orcid.org/0000-0002-9913-1144
Takeshi IshimizuCollege of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan.ORCID http://orcid.org/0000-0003-4910-6810
Masami KusunokiProfessor Emeritus, University of Yamanashi, Kofu, Yamanashi, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In 1935, Shiro Akabori began research on the preparation of taka-amylase A with a purity suitable for chemical research, with the intention of elucidating the chemical nature of the enzyme. He succeeded in developing a method to efficiently obtain crystallized taka-amylase A from Aspergillus oryzae. Using crystallized taka-amylase A as the starting material, a series of studies were conducted to determine its amino acid composition and sequence, sugar chain structure, and three-dimensional structure. Based on these results, the molecular structure and catalytic mechanism of taka-amylase A were elucidated. The scientific achievements from research on taka-amylase A significantly enhanced Japan's capabilities in protein research, represented by the fact that taka-amylase A was the first amylase in the world for which both chemical and crystallographic structures were elucidated.

Indexed as

alpha-AmylasesCrystallizationAmino Acid SequenceAspergillus oryzaeCrystallography, X-RayJapanalpha-Amylasesenzymeprotein chemistryprotein structureShiro Akaborisugar chain analysistaka-amylase A

Identifiers

PMID39401898
PMCPMC11535007

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