ArticleProceedings of the National Academy of Sciences of the United States of America2024
Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.Biomedical optics express · 2025Article
- hyperPICASSO: adapting the mutual-information-based unmixing technique PICASSO to hyperspectral datasets.Optics letters · 2025Article
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4 authors.
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Abstract
Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named [Formula: see text]-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its function, we use [Formula: see text]-OPM to map and identify microplastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that [Formula: see text]-OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and extracellular matrix composition in the wounded region. Finally, we use [Formula: see text]-OPM to synchronize and average 36,800 individual frames to obtain hyperspectral videos of a zebrafish embryo's beating heart at an effective 28 frames per second, recording compositional changes throughout the cardiac cycle.
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