Evidence map›Paper›PMID 39400682›Full record

ArticleMedical oncology (Northwood, London, England)2024

Glycolytic pathway analysis and gene expression profiles of combination of aloe vera and paclitaxel on non-small cell lung cancer and breast cancer.

Tuğba Kul Köprülü, Jülide Balkan, Bahar Gezer, Burçin Erkal Çam

Abstract read
PubMed Publisher
In one paragraph

Article in Medical oncology (Northwood, London, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tuğba Kul KöprülüExperimental Medicine Application and Research Center, Validebağ Research Park, University of Health Sciences, Altunizade, Kalfaçeşme Street, Üsküdar, 34622, Istanbul, Turkey. tugbakul.koprulu@sbu.edu.tr.ORCID http://orcid.org/0000-0001-9451-5715
Jülide BalkanDepartment of Molecular Medicine, Hamidiye Institute of Health Sciences, University of Health Sciences, 34622, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-9690-5220
Bahar GezerDepartment of Molecular Medicine, Hamidiye Institute of Health Sciences, University of Health Sciences, 34622, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-2054-0034
Burçin Erkal ÇamDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yıldız Technical University, Esenler, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-0275-5613

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this study is to enhance the effectiveness of known anticancer medications using natural compounds. The study investigated the impact of combining AVE with PAX on non-small cell lung cancer (A549) and breast cancer (MCF7). In this study, A549 and MCF7 cells were treated with PAX (5 μM), AVE (24 μg/mL), and a combination of PAX and AVE (5 μM + 24 μg/mL). The glucose consumption rates of the cells were determined by extracellular acidification rate (ECAR) thanks to the SeaHorse XFe24 instrument. In addition, gene expression profiles were determined by performing Total RNA sequencing with the Novaseq 6000 instrument. Finally, the expressions of GAPDH, BAX, and BCL-2 genes involved in the apoptotic pathway were detected by RT-qPCR. The combined application of PAX and AVE reduced the ECAR value in both cell lines. According to the RT-qPCR results, the expression level of the apoptotic gene BAX increased in both cell lines (p < 0.05). Total RNA sequencing revealed that the combination effects of PAX and AVE play a role in the ribosome mechanism, thereby affecting the protein translation system in MCF7 while apoptosis and cell cycle have come to the forefront in A549.

Indexed as

Breast NeoplasmsCarcinoma, Non-Small-Cell LungLung NeoplasmsPaclitaxelA549 CellsApoptosisFemaleGene Expression Regulation, NeoplasticGlycolysisHumansMCF-7 CellsPlant ExtractsTranscriptomePaclitaxelPlant ExtractsAloe veraBreast cancerNon-small cell lung cancerPaclitaxelSeaHorse XFe24 analyzerTotal RNA sequencing

Identifiers

PMID39400682

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.