Evidence map›Paper›PMID 39400454›Full record

ArticleJournal of extracellular vesicles2024

Size photometry and fluorescence imaging of immobilized immersed extracellular vesicles.

Andreas Wallucks, Philippe DeCorwin-Martin, Molly L Shen, Andy Ng, David Juncker

Abstract read
In one paragraph

Article in Journal of extracellular vesicles, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andreas WallucksBiomedical Engineering Department, McGill University, Montreal, QC, Canada.ORCID 0000-0001-7177-1759
Philippe DeCorwin-MartinBiomedical Engineering Department, McGill University, Montreal, QC, Canada.ORCID 0000-0001-8132-1102
Molly L ShenBiomedical Engineering Department, McGill University, Montreal, QC, Canada.ORCID 0000-0003-3383-619X
Andy NgBiomedical Engineering Department, McGill University, Montreal, QC, Canada.ORCID 0000-0003-3257-0801
David JunckerBiomedical Engineering Department, McGill University, Montreal, QC, Canada.ORCID 0000-0002-7313-1162

Funding

Canada Research ChairsCancer Research Society 943871Genome Canada Disruptive Innovation in Genomics 15502Natural Sciences and Engineering Research Council of Canada RGPIN-2016-06723Schmidt Science Fellows
6 · The paper itself

Abstract

Immunofluorescence analysis of individual extracellular vesicles (EVs) in common fluorescence microscopes is gaining popularity due to its accessibility and high fluorescence sensitivity; however, EV number and size are only measurable using fluorescent stains requiring extensive sample manipulations. Here we introduce highly sensitive label-free EV size photometry (SP) based on interferometric scattering (iSCAT) imaging of immersed EVs immobilized on a glass coverslip. We implement SP on a common inverted epifluorescence microscope with LED illumination and a simple 50:50 beamsplitter, permitting seamless integration of SP with fluorescence imaging (SPFI). We present a high-throughput SPFI workflow recording >10,000 EVs in 7 min over ten 88 × 88 µm

Indexed as

Extracellular VesiclesFlow CytometryHumansMicroscopy, FluorescenceNanoparticlesOptical ImagingParticle SizePhotometrySilicon DioxideSilicon Dioxideextracellular vesiclesinterferometric scattering microscopylimit of detectionsingle vesicle analysissize

Identifiers

PMID39400454
PMCPMC11472239

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.