Evidence map›Paper›PMID 39399693›Full record

ArticleMolecular breeding : new strategies in plant improvement2024

Development and application of Key Allele-Specific PCR (KASP) molecular markers for assessing apple fruit crispness.

Yao Xiao, Ling Wu, Baoan Wang, Manyu Zhang, Qi Pan, Linfeng Xian, Jing Sheng, Mengbo Yan, Jingxian Jin, Rui Zhang and 4 more

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Article in Molecular breeding : new strategies in plant improvement, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Fruit crops: development, stress resilience and molecular breeding.Molecular breeding : new strategies in plant improvement · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yao Xiao *Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Ling Wu *Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Baoan WangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Manyu ZhangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Qi PanLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Linfeng XianLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Jing ShengLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Mengbo YanLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Jingxian JinLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Rui ZhangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Jing ZhangLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Qiulin ZengLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Tianzhong LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.
Wei LiLaboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193 China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Crispness stands as a pivotal criterion in assessing apple texture, widely cherished by consumers. Yet, owing to its multifaceted nature, crispness remains a formidable challenge in artificial enhancement efforts. To expedite the early and precise evaluation of apple crispness, this study centered on a hybrid population derived from 'Fuji' and 'Pink Lady' cultivars, showcasing segregating crispness traits. We conducted measurements of flesh water content, cellular anatomical morphology, and employed a texture analyzer to assess mechanical properties of the offspring flesh. Integrating these three dimensions, we conducted a comprehensive analysis of quantitative characteristics of apple crispness, juxtaposed with sensory evaluation. Utilizing BSA-seq technology, we scrutinized extreme phenotypic individuals, revealing QTL loci intricately linked to the aforementioned dimensions, and subsequently developed Key Allele-Specific PCR (KASP) markers. These markers underwent validation in hybrid populations of 'Hanfu' x 'Pink Lady' and 'Hanfu' x 'Honey Crisp'. Our findings underscored significant correlations between mechanical properties, water content, and cell size with crispness. Higher mechanical properties and water content, alongside smaller cell size, correlated with firmer flesh texture; moderate mechanical properties, and elevated water content and cell size, with crisper texture; whereas lower mechanical properties, water content, and cell size implied softer flesh.The study yielded KASP markers effectively reflecting flesh mechanical properties (SNP_24399345), water content (SNP_8667563), and cell size (SNP_15566229). Comprehensive analysis of these markers identified CC-CC-TT as an effective identifier of soft flesh individuals; while GG-TC-TT and GG-CC-TT combinations better represented individuals with harder flesh. The Crunchy subclass could be discerned by combinations of GG-TC-TC, GG-TC-CC, GG-TT-TC, and GG-TT-CC. These findings furnish effective molecular markers for the genetic enhancement of apple crispness, bearing significant implications for the cultivation of novel apple varieties. Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-024-01509-1.

Indexed as

AppleBSA-seqCrispnessKASPMarker combination

Identifiers

PMID39399693
PMCPMC11467153

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