Evidence map›Paper›PMID 39398600›Full record

ArticleAmerican journal of translational research2024

Three-dimensional visualization of Hertwig's epithelial root sheath during tooth root development with the miniTESOS tissue clearing method.

Jiayu Wang, Miao Yu, Yang Liu, Dong Han

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Article in American journal of translational research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Jiayu WangDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Central Laboratory, Peking University School and Hospital of Stomatology No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.
Miao YuDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Central Laboratory, Peking University School and Hospital of Stomatology No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.
Yang LiuDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Central Laboratory, Peking University School and Hospital of Stomatology No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.
Dong HanDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Central Laboratory, Peking University School and Hospital of Stomatology No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveHertwig's epithelial root sheath (HERS) acts as a signaling center that regulates the size, shape, and number of tooth roots. Therefore, understanding the anatomical changes in HERS during development is crucial for investigating its effect on root formation. However, the three-dimensional morphology of HERS and its changes during tooth root development remain largely unknown due to the limitations of traditional histological techniques.

methodsWe developed an improved tissue clearing method for mouse embryonic and early postnatal mandibles, designated as a mini Transparent Embedding Solvent System (miniTESOS), based on the Transparent Embedding Solvent System (TESOS). We applied this method to the

resultsIn the early stages of root development, differences in growth rates and developmental patterns between MM1 and MM2 were observed in the mesiodistal and buccolingual directions, from the initiation to the fusion of buccal and lingual HERS. In the elongation stage of two-rooted teeth, continuous HERS was found exclusively at the leading edge of the root, gradually decreasing in length as the root extended. In the root bifurcation area, HERS undergoes four developmental stages: initiation, elongation, contact, and complete fragmentation, each characterized by specific morphological features.

conclusionThis study improves the understanding of the alterations in HERS during root development and summarizes its developmental pattern in the root bifurcation region. The miniTESOS tissue clearing method provides a new strategy to investigate tooth development.

Indexed as

Hertwig’s epithelial root sheathminiTESOSTESOSthree-dimensional imagingtissue clearingtooth root development

Identifiers

PMID39398600
PMCPMC11470359

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