Evidence map›Paper›PMID 39397178›Full record

ArticleMikrochimica acta2024

Electrochemiluminescence biosensor for MMP-2 determination using CRISPR/Cas13a and EXPAR amplification: a novel approach for anti-aging research.

Qiang Tang, Jie Wang, Jiayi Zhang, Hongyu Zeng, Zhixue Su, Xiying Zhu, Jihua Wei, Yuanxun Gong, Qianli Tang, Kai Zhang and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qiang TangGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Jie WangGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Jiayi ZhangWest Guangxi Key Laboratory for Prevention and Treatment of High-Incidence Diseases, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Hongyu ZengGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Zhixue SuGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Xiying ZhuGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Jihua WeiGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Yuanxun GongGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
Qianli TangGuangxi Key Laboratory for Preclinical and Translational Research On Bone and Joint Degenerative Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. htmgx919@163.com.
Kai ZhangSchool of Chemistry and Materials Science, Nanjing University of Information Science and Technology, Nanjing, 210044, P. R. China. kaizhang@nuist.edu.cn.
Xianjiu LiaoWest Guangxi Key Laboratory for Prevention and Treatment of High-Incidence Diseases, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. lxj2006910@163.com.

Funding

financial support of the National Natural Science Foundation of China 21964018Startup Foundation for Introducing Talent of Nanjing University of Information Science and Technology 2023r129
6 · The paper itself

Abstract

Matrix metalloproteinase-2 (MMP-2) plays a pivotal role in anti-aging research. Developing advanced detection platforms for MMP-2 with high specificity, sensitivity, and accessibility is crucial. This study introduces a novel electrochemiluminescence (ECL) biosensor for MMP-2 determination, leveraging the CRISPR/Cas13a system and Exponential Amplification Reaction (EXPAR). The biosensor operates by utilizing the T7 RNA polymerase to transcribe RNA from a DNA template upon MMP-2 interaction. This RNA activates Cas13a, leading to signal amplification and ECL detection. The incorporation of the "photoswitch" molecule [Ru(phen)

Indexed as

Biosensing TechniquesCRISPR-Cas SystemsElectrochemical TechniquesLimit of DetectionLuminescent MeasurementsMatrix Metalloproteinase 2HumansNucleic Acid Amplification TechniquesReproducibility of ResultsMatrix Metalloproteinase 2MMP2 protein, humanCRISPR/Cas13aElectrochemiluminescenceMMP-2T7 RNA

Identifiers

PMID39397178

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.