Evidence map›Paper›PMID 39396232›Full record

ArticleSTAR protocols2024

Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform.

Meilan Fu, Xiangwei Wang, Shubing Tang, Shimeng Bai, Longfei Ding, Yangyang Hu, Kangli Cao, Tianhan Yang, Chen Zhao, Xiaoyan Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Meilan FuShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Xiangwei WangShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Shubing TangShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Department of Investigational New Drug, Shanghai Reinovax Biologics Co., Ltd., Shanghai, China.
Shimeng BaiShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Bio-therapeutic Center, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, The Second Hospital Affiliated with the School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Longfei DingShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Yangyang HuShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Kangli CaoZhongshan Hospital, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Tianhan YangShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Chen ZhaoShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. Electronic address: chen_zhao72@163.com.
Xiaoyan ZhangShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Zhongshan Hospital, Institutes of Biomedical Sciences, Fudan University, Shanghai, China; Xiamen Key Laboratory of Biotherapy, Xiammen, China; Clinical Center for Biotherapy, Zhongshan Hospital(Xiamen), Fudan University, Xiamen, China. Electronic address: zhangxiaoyan@fudan.edu.cn.
Jianqing XuShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Zhongshan Hospital, Institutes of Biomedical Sciences, Fudan University, Shanghai, China; Xiamen Key Laboratory of Biotherapy, Xiammen, China; Clinical Center for Biotherapy, Zhongshan Hospital(Xiamen), Fudan University, Xiamen, China. Electronic address: xujianqing@fudan.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, we present a protocol for isolating antigen-specific plasma B cells from immunized mice using Beacon, sequencing the encoded B cell receptors (BCRs), and cloning and expressing the resulting antibodies. This protocol can easily be extended to human samples.

Indexed as

Antibodies, MonoclonalAnimalsAntigensB-LymphocytesFlow CytometryHumansImmunizationMicePlasma CellsReceptors, Antigen, B-CellAntibodies, MonoclonalAntigensReceptors, Antigen, B-CellAntibodyCell isolationProtein expression and purificationSequencingSingle Cell

Identifiers

PMID39396232
PMCPMC11735987

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.