Evidence map›Paper›PMID 39395241›Full record

ArticleRedox biology2024

Effect of plasma-induced oxidation on NK cell immune checkpoint ligands: A computational-experimental approach.

Pepijn Heirman, Hanne Verswyvel, Mauranne Bauwens, Maksudbek Yusupov, Jorrit De Waele, Abraham Lin, Evelien Smits, Annemie Bogaerts

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pepijn HeirmanResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium. Electronic address: pepijn.heirman@uantwerpen.be.
Hanne VerswyvelResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium; Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610, Antwerp, Wilrijk, Belgium. Electronic address: hanne.verswyvel@uantwerpen.be.
Mauranne BauwensResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium; Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610, Antwerp, Wilrijk, Belgium.
Maksudbek YusupovResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium; Institute of Fundamental and Applied Research, National Research University TIIAME, 100000, Tashkent, Uzbekistan; Laboratory of Experimental Biophysics, Center for Advanced Technologies, 100174, Tashkent, Uzbekistan.
Jorrit De WaeleCenter for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610, Antwerp, Wilrijk, Belgium.
Abraham LinResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium; Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610, Antwerp, Wilrijk, Belgium.
Evelien SmitsCenter for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610, Antwerp, Wilrijk, Belgium.
Annemie BogaertsResearch Group PLASMANT, Department of Chemistry, University of Antwerp, 2610, Antwerp, Wilrijk, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-thermal plasma (NTP) shows promise as a potent anti-cancer therapy with both cytotoxic and immunomodulatory effects. In this study, we investigate the chemical and biological effects of NTP-induced oxidation on several key, determinant immune checkpoints of natural killer (NK) cell function. We used molecular dynamics (MD) and umbrella sampling simulations to investigate the effect of NTP-induced oxidative changes on the MHC-I complexes HLA-Cw4 and HLA-E. Our simulations indicate that these chemical alterations do not significantly affect the binding affinity of these markers to their corresponding NK cell receptor, which is supported with experimental read-outs of ligand expression on human head and neck squamous cell carcinoma cells after NTP application. Broadening our scope to other key ligands for NK cell reactivity, we demonstrate rapid reduction in CD155 and CD112, target ligands of the inhibitory TIGIT axis, and in immune checkpoint CD73 immediately after treatment. Besides these transient chemical alterations, the reactive species in NTP cause a cascade of downstream cellular reactions. This is underlined by the upregulation of the stress proteins MICA/B, potent ligands for NK cell activation, 24 h post treatment. Taken together, this work corroborates the immunomodulatory potential of NTP, and sheds light on the interaction mechanisms between NTP and cancer cells.

Indexed as

Killer Cells, NaturalMolecular Dynamics SimulationOxidation-ReductionCell Line, TumorHistocompatibility Antigens Class IHumansImmune Checkpoint ProteinsLigandsReceptors, VirusHistocompatibility Antigens Class IImmune Checkpoint ProteinsLigandspoliovirus receptorReceptors, VirusCancer immunotherapyImmune checkpointsNatural killer cellsNon-thermal plasmaOxidative stressUmbrella sampling

Identifiers

PMID39395241
PMCPMC11663777

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.