Evidence map›Paper›PMID 39395097›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor.

Aqfan Jamaluddin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Aqfan JamaluddinMetabolism and Systems Science, University of Birmingham, Birmingham, UK. a.jamaluddin@bham.ac.uk.

Funding

Wellcome Trust 224155/Z/21/ZWellcome Trust SBF004|1034
6 · The paper itself

Abstract

This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET). Focusing on the eBRET

Indexed as

Bioluminescence Resonance Energy Transfer TechniquesGTP-Binding Protein alpha Subunits, Gq-G11Receptors, GhrelinHEK293 CellsHumansProtein BindingSignal TransductionTransfectionGTP-Binding Protein alpha Subunits, Gq-G11Receptors, GhrelinCoelenterazine-HFurimazineG proteinG protein recruitmentProtein interactionsReceptor activityRenilla luciferase

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.