ArticleNature methods2024
Single-shot 20-fold expansion microscopy.
Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- ONE microscopy.Nature protocols · 2026Review
- Hydrogel Chemistry in Expansion Microscopy: From Polyacrylamide to Next-Generation Swellable Polymers.Chembiochem : a European journal of chemical biology · 2026Review
- Thousandfold Expansion Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Multi-Tiered µDicer Enables Protein-Preserving Microdissection at 10 µm Resolution.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Article
- Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples.Nature protocols · 2026Review
- HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.Cell reports methods · 2026Article
- Machine Learning in Single-Molecule Tracking Analysis of Superresolution Optical Microscopy Data.Cells · 2026Review
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- Functional super-resolution microscopy of fibers and polymers: convergence of artificial and biological systems at the nanoscale.Nanoscale horizons · 2026Review
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Seeing the Unseen: Super-Resolution Microscopy in Protein Aggregation Research.Chemical & biomedical imaging · 2026Review
- Tensile expansion microscopy applies mechanical force to super-resolve fixed and image live cellular samples.bioRxiv : the preprint server for biology · 2026Article
- Synaptic-resolution connectomics: towards large brains and connectomic screening.Nature reviews. Neuroscience · 2026Review
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Principles ofbioRxiv : the preprint server for biology · 2026Article
- Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure.bioRxiv : the preprint server for biology · 2026Article
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Applications and development of in situ nucleic acid visualization techniques.Frontiers in bioengineering and biotechnology · 2026Review
- Visualizing Drosophila centrioles by expansion microscopy.Journal of cell science · 2026Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (~4-10× linearly) or achieve larger expansion factors through iterating the expansion process a second time (~15-20× linearly). Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.