Evidence map›Paper›PMID 39394068›Full record

ArticleBMC plant biology2024

Transcriptional landscape of sweetpotato root tip development at the single-cell level.

Nan Zhao, Xiawei Ding, CaiHuan Tian, Shixin Wang, Shuyan Xie, Hongda Zou, Hao Liu, Jingyi Chen, Xue Lian Liang, Lifei Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Structural Variation and Its Roles in Plant Genomes.Plants (Basel, Switzerland) · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nan Zhao *Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Xiawei Ding *Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
CaiHuan TianInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Shixin WangCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Shuyan XieCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Hongda ZouCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Hao LiuCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Jingyi ChenCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China.
Xue Lian LiangCollege of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510000, Guangdong, China. liangxuelian2005@sina.com.
Lifei HuangCrop Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou, 510000, Guangdong, China. hlf157@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell transcriptome sequencing (scRNA-seq) is a powerful tool for describing the transcriptome dynamics of plant development but has not yet been utilized to analyze the tissue ontology of sweetpotato. This study established a stable method for isolating single protoplast cells for scRNA-seq to reveal the cell heterogeneity of sweetpotato root tip meristems at the single-cell level. The study analyzed 12,172 single cells and 27,355 genes in the root tips of the sweetpotato variety Guangshu 87, which were distributed into 15 cell clusters. Pseudo-time analysis showed that there were transitional cells in the apical development trajectory of mature cell types from stem cell niches. Furthermore, we identified novel development regulators of sweetpotato tubers via trajectory analysis. The transcription factor IbGATA4 was highly expressed in the adventitious roots during the development of sweetpotato root tips, where it may regulate the development of sweetpotato root tips. In addition, significant differences were observed in the transcriptional profiles of cell types between sweetpotato, Arabidopsis thaliana, and maize. This study mapped the single-cell transcriptome of sweetpotato root tips, laying a foundation for studying the types, functions, differentiation, and development of sweetpotato root tip cells.

Indexed as

Ipomoea batatasMeristemSingle-Cell AnalysisArabidopsisGene Expression ProfilingGene Expression Regulation, PlantPlant ProteinsPlant RootsTranscription FactorsTranscriptomeZea maysPlant ProteinsTranscription FactorsDevelopmental trajectoryIpomoea batatas (L.) Lam.RootscRNA-seqTransitional cells

Identifiers

PMID39394068
PMCPMC11475360

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.