Evidence map›Paper›PMID 39392130›Full record

ArticleBiotechnology and bioengineering2025

Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis.

Jacob M Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S Clark, Jeffrey M Skerker, Adam P Arkin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jacob M HilzingerDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.ORCID 0000-0002-1876-1313
Skyler FriedlineDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.
Divya SivanandanDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.
Ya-Fang ChengQB3-Berkeley, University of California, Berkeley, California, USA.
Shunsuke YamazakiDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.
Douglas S ClarkDepartment of Chemical and Biomolecular Engineering, University of California-Berkeley, Berkeley, California, USA.
Jeffrey M SkerkerDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.ORCID 0000-0003-2653-1566
Adam P ArkinDepartment of Biological Engineering, University of California-Berkeley, Berkeley, California, USA.ORCID 0000-0002-4999-2931

Funding

Pacific Biosciences PacBio RS Single Molecule Real Time SequencerS10OD018174 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI ROKHSAR, DANIEL SOLEYMAN · 2014 to 2014
$600k
NASANIH HHS S10 OD018174
6 · The paper itself

Abstract

Spirulina is the common name for the edible, nonheterocystous, filamentous cyanobacterium Arthrospira platensis that is grown industrially as a food supplement, animal feedstock, and pigment source. Although there are many applications for engineering this organism, until recently no genetic tools or reproducible transformation methods have been published. While recent work showed the production of a diversity of proteins in A. platensis, including single-domain antibodies for oral delivery, there remains a need for a modular, characterized genetic toolkit. Here, we independently establish a reproducible method for the transformation of A. platensis and engineer this bacterium to produce acetaminophen as proof-of-concept for small molecule production in an edible host. This work opens A. platensis to the wider scientific community for future engineering as a functional food for nutritional enhancement, modification of organoleptic traits, and production of pharmaceuticals for oral delivery.

Indexed as

AcetaminophenSpirulinaMetabolic EngineeringAcetaminophenArthrospira platensiscyanobacteriaspirulina

Identifiers

PMID39392130
PMCPMC11632167

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.