ArticlemicroPublication biology2024
Methodology to enable high-throughput imaging of Arabidopsis seedlings on cover glass-bottom multiwell plates.
Article in microPublication biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
High-throughput imaging enables rapid collection of large datasets and is used widely in many systems. However, this is not often used in plant-based systems due to issues related to the need to mount tissues and autofluorescence of plant metabolites. We therefore developed methodology enabling high-throughput imaging of Arabidopsis roots. In this system, growth media supplemented with India Ink (to block autofluorescence from cotyledons) is poured directly into multi-well coverglass-bottom plates and seedlings grown such that the roots grow down with the gravity vector and along the coverglass, effectively mounting themselves for imaging. This method enables high-throughput imaging of Arabidopsis roots.
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