Evidence map›Paper›PMID 39388051›Full record

ArticleActa parasitologica2024

The Role of Dicrocoelium dendriticum Egg Antigen in Colitis: A Molecular, Pathological and Serological Study in an Experimental Model of C57BL/6 Mice.

Malihe Eilakinezhad, Leila Mighani, Majid Khazaei, Seyed Alireza Esmaeili, Seyedeh Elnaz Nazari, Moein Eskandari, Seyed Mojtaba Mousavi Bazzaz, Khatereh Kharazmi, Elham Moghaddas, Mehdi Zarean

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Article in Acta parasitologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Malihe Eilakinezhad *Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Leila Mighani *Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Majid KhazaeiDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Seyed Alireza EsmaeiliImmunology Department, Mashhad University of Medical Sciences, Mashhad, Iran.
Seyedeh Elnaz NazariDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Moein EskandariDepartment of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Seyed Mojtaba Mousavi BazzazDepartment of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Khatereh KharazmiDepartment of Physiology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Elham MoghaddasDepartment of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Elham.moghaddas@monash.edu.
Mehdi ZareanDepartment of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. ZareanM@mums.ac.ir.

Funding

Mashhad University of Medical Sciences 4001731
6 · The paper itself

Abstract

backgroundInflammatory bowel disease (IBD) is a chronic and recurrent disease of the gastrointestinal tract that enhances the chance of developing colorectal cancer. Since standard treatments such as Mesalazine have limited effectiveness and are often accompanied by numerous side effects, the use of immune modulators derived from worms has been proposed as a new immunotherapy method for inflammatory diseases such as ulcerative colitis. The aim of this study is to investigate the protective effects of D. dendriticum egg antigen on DSS-induced colitis in C57BL/6 mice.

methodsD. dendriticum egg antigen was extracted and DSS (3.5%) was used to induce colitis in mice. Treatment and prophylaxis included intraperitoneal injections of D. dendriticum egg antigen. Histopathological indicators and the disease activity index (DAI), including weight loss, rectal bleeding, stool consistency, and rectal prolapse, were used to assess the severity of colitis. Real-time PCR measured the expression of transforming growth factor-β (TGF-β) and interleukin-17 (IL-17), while ELISA determined the concentration of these cytokines.

resultsTreatment with D. dendriticum egg antigen significantly improved the clinical symptoms and decreased the severity of DSS-induced colitis. Furthermore, D. dendriticum egg antigen increased the expression of TGF-β mRNA and reduced the expression of IL-17 mRNA, leading to a positive adjustment in the regulation of proteins and reduction of inflammatory proteins. As a result, the macroscopic, microscopic inflammation and activity index (DAI) of DSS-induced decreased.

conclusionD. dendriticum egg antigen provides a promising new way to modulate the immune system and improve ulcerative colitis.

Indexed as

Antigens, HelminthColitisDextran SulfateDicrocoeliumDisease Models, AnimalMice, Inbred C57BLAnimalsFemaleInterleukin-17MiceTransforming Growth Factor betaAntigens, HelminthDextran SulfateInterleukin-17Transforming Growth Factor betaColitisDicrocoelium dendriticum eggIL-17MesalazineTGF-βUlcerative

Identifiers

PMID39388051

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.