ArticleMolecular ecology resources2025
Barcode 100K Specimens: In a Single Nanopore Run.
Article in Molecular ecology resources, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- A Genomic Tool to Tackle Cryptic Diversity Demonstrates the Potential for Off-Target Use of GT-Seq Panels.Molecular ecology resources · 2026Article
- AI-driven technological breakthroughs and practical pathways for biodiversity conservation and ecological management.iScience · 2026Review
- Beyond conventional pollinators: advancing diversity assessments of neglected insect taxa.Annals of the Entomological Society of America · 2026Article
- Species identification, discovery, and biomonitoring: Strategic priorities for DNA barcoding in Europe, set in a global context.Bioscience · 2026Article
- A Multilocus Nanopore eDNA Workflow for Red Algal Diversity Assessment in an Algal Reef System.Ecology and evolution · 2026Article
- High-Throughput DNA Barcoding Reveals Multi-Trophic Networks of Plants, Leafminers and Parasitoids in a Tropical Dry Forest.Molecular ecology resources · 2026Article
- Phylogenetic Authentication of Amplicon Sequence Variants in Single-Specimen Metabarcoding of Tropical Insects.Molecular ecology resources · 2026Article
- ReadChop: a high-performance demultiplexer for long-read sequencing data.Bioinformatics (Oxford, England) · 2026Article
- Revisions to theMycoKeys · 2026Article
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- Article
- DNA barcoding of Austrian terrestrial isopods (Crustacea: Isopoda: Oniscidea) reveals potential cryptic diversity.PloS one · 2026Article
- A synopsis of neotropical parasitoid wasps ofZooKeys · 2026Article
- An All-in-One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance.Molecular ecology resources · 2025Article
- A Comprehensive DNA Barcode Reference Library for the Macroinvertebrates of Scottish Seagrass Beds Using Oxford Nanopore Flongle Flowcells.Ecology and evolution · 2025Article
- Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa.Scientific data · 2025Article
- Incorporating Neglected Insect Larvae in Species Inventories: DNA Barcoding as an Effective Tool for All-Stage Invertebrate Identification in Tree Holes.Ecology and evolution · 2025Article
- Gaps and Data Ambiguities in DNA Reference Libraries: A Limiting Factor for Molecular-Based Biodiversity Assessments Using Annelids as a Case Study.Ecology and evolution · 2025Article
- Delimiting Species-Prospects and Challenges for DNA Barcoding.Molecular ecology · 2025Article
- Rapid on-site universal vertebrate species identification via multi-barcode nanopore sequencing.PloS one · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
It is a global priority to better manage the biosphere, but action must be informed by comprehensive data on the abundance and distribution of species. The acquisition of such information is currently constrained by high costs. DNA barcoding can speed the registration of unknown animal species, the most diverse kingdom of eukaryotes, as the BIN system automates their recognition. However, inexpensive sequencing protocols are critical as the census of all animal species is likely to require the analysis of a billion or more specimens. Barcoding involves DNA extraction followed by PCR and sequencing with the last step dominating costs until 2017. By enabling the sequencing of highly multiplexed samples, the Sequel platforms from Pacific BioSciences slashed costs by 90%, but these instruments are only deployed in core facilities because of their expense. Sequencers from Oxford Nanopore Technologies provide an escape from high capital and service costs, but their low sequence fidelity has, until recently, constrained adoption. However, the improved performance of its latest flow cells (R10.4.1) erases this barrier. This study demonstrates that a MinION flow cell can characterise an amplicon pool derived from 100,000 specimens while a Flongle flow cell can process one derived from several thousand. At $0.01 per specimen, DNA sequencing is now the least expensive step in the barcode workflow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.