Evidence map›Paper›PMID 39386543›Full record

ArticlebioRxiv : the preprint server for biology2024

Genomic mosaicism reveals developmental organization of trunk neural crest-derived ganglia.

Keng Ioi Vong, Yanina D Alvarez, Geoffroy Noel, Scott T Barton, Changuk Chung, Robyn Howarth, Naomi Meave, Qingquan Zhang, Fiza Jiwani, Chelsea Barrows and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Keng Ioi VongDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.ORCID 0000-0001-5377-9845
Yanina D AlvarezInstitute for Molecular Biosciences, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
Geoffroy NoelDivision of Medical Education, School of Medicine, University of California San Diego, La Jolla, CA 92037, USA.
Scott T BartonDivision of Medical Education, School of Medicine, University of California San Diego, La Jolla, CA 92037, USA.
Changuk ChungDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Robyn HowarthDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Naomi MeaveDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Qingquan ZhangDivision of Cardiology, Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA.
Fiza JiwaniDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Chelsea BarrowsDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Arzoo PatelDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.
Jiang Xiong WangInstitute for Molecular Biosciences, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
Neil ChiDivision of Cardiology, Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA.
Stephen F KingsmoreRady Children's Institute for Genomic Medicine, San Diego, CA 92123, USA.ORCID 0000-0001-7180-2527
Melanie D WhiteInstitute for Molecular Biosciences, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.ORCID 0000-0002-7399-8348
Xiaoxu YangDepartment of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.ORCID 0000-0003-0219-0023
Joseph G GleesonDepartment of Neurosciences, University of California San Diego, La Jolla, CA 92037, USA.ORCID 0000-0002-0889-9220

Funding

Project II - Modeling meningomyelocele in frog using human alleles and folic acid exposureP01HD104436 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ECKER, JOSEPH R · 2021 to 2025
$7.0M
Mosaicism in focal cortical dysplasias spectrum seen in neuropsychiatric diseaseU01MH108898 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G · 2015 to 2019
$5.1M
Origins of Brain Somatic Mosaicism in Developmental Brain DiseaseR01MH124890 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G · 2021 to 2025
$1.5M
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
Understanding genomic stability between generations by assessing mutational burdens in single spermsR00HD111686 · NICHD · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Xiaoxu Yang · 2025 to 2026
$498k
From genomics to natural language processing: A protected environment for research computing in the health scienceS10OD021644 · OD · UNIVERSITY OF UTAH · PI CHEATHAM, THOMAS E. · 2017 to 2017
$494k
Mechanisms controlling HIV latency and pathogenesis in microgliaR21MH134401 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHLACHETZKI, JOHANNES CAROLUS MAGNUS, YANG, XIAOXU · 2023 to 2023
$435k
Understanding genomic stability betweengenerations by assessing mutational burdens in single spermsK99HD111686 · NICHD · UNIVERSITY OF UTAH · PI YANG, XIAOXU · 2023 to 2024
$232k
NICHD NIH HHS K99 HD111686NICHD NIH HHS P01 HD104436NICHD NIH HHS R00 HD111686NIH HHS S10 OD021644NIH HHS S10 OD026929NIMH NIH HHS R01 MH124890NIMH NIH HHS R21 MH134401NIMH NIH HHS U01 MH108898
6 · The paper itself

Abstract

The neural crest generates numerous cell types, but conflicting results leave developmental origins unresolved. Here using somatic mosaic variants as cellular barcodes, we infer embryonic clonal dynamics of trunk neural crest, focusing on the sensory and sympathetic ganglia. From three independent adult neurotypical human donors, we identified 1,278 mosaic variants using deep whole-genome sequencing, then profiled allelic fractions in 187 anatomically dissected ganglia. We found a massive rostrocaudal spread of progenitor clones specific to sensory or sympathetic ganglia, which unlike in the brain, showed robust bilateral distributions. Computational modeling suggested neural crest progenitor fate specification preceded delamination from neural tube. Single-cell multiomic analysis suggested both neurons and glia contributed to the rostrocaudal clonal organization. CRISPR barcoding in mice and live imaging in quail embryos confirmed these clonal dynamics across multiple somite levels. Our findings reveal an evolutionarily conserved clonal spread of cells populating peripheral neural ganglia.

Indexed as

cell migrationdorsal root ganglialineage tracinglive imagingneural crestsomatic mosaicismsympathetic ganglia

Identifiers

PMID39386543
PMCPMC11463384

What OpenQuestion holds

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LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.