Evidence map›Paper›PMID 39386506›Full record

ArticlebioRxiv : the preprint server for biology2025

TWINKLE: An open-source two-photon microscope for teaching and research.

Manuel Schottdorf, P Dylan Rich, E Mika Diamanti, Albert Lin, Sina Tafazoli, Edward H Nieh, Stephan Y Thiberge

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Manuel SchottdorfPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0002-5468-4255
P Dylan RichPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0001-9782-7984
E Mika DiamantiPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0003-1199-3362
Albert LinPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0002-4541-5889
Sina TafazoliPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0003-1926-0227
Edward H NiehPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0003-2154-6224
Stephan Y ThibergePrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USA.ORCID 0000-0002-6583-6613

Funding

P5: Mechanistic Multi-Region Brain ModelsU19NS132720 · NINDS · PRINCETON UNIVERSITY · PI Carlos D Brody, Ila R. Fiete · 2023 to 2026
$21.5M
NINDS NIH HHS U19 NS132720
6 · The paper itself

Abstract

Many laboratories use two-photon microscopy through commercial suppliers, or homemade designs of considerable complexity. The integrated nature of these systems complicates customization, troubleshooting, and training on the principles of two-photon microscopy. Here, we present "Twinkle": a microscope for Two-photon Imaging in Neuroscience, and Kit for Learning and Education. It is a fully open, high performing and easy-to-set-up microscope that can effectively be used for both education and research. The instrument features a > 1 mm field of view, using a modern objective with 3 mm working distance and 2 inch diameter optics combined with GaAsP photomultiplier tubes to maximize the fluorescence signal. We document our experiences using this system as a teaching tool in several two week long workshops, exemplify scientific use cases, and conclude with a broader note on the place of our work in the growing space of open scientific instrumentation.

Identifiers

PMID39386506
PMCPMC11463478

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.