Evidence map›Paper›PMID 39386460›Full record

ArticlebioRxiv : the preprint server for biology2024

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus.

S A Quinodoz, L Jiang, A A Abu-Alfa, T J Comi, H Zhao, Q Yu, L W Wiesner, J F Botello, A Donlic, E Soehalim and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

S A QuinodozDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0003-1862-5204
L JiangDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0009-0007-7291-8311
A A Abu-AlfaDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
T J ComiOmenn-Darling Bioengineering Institute, Princeton University, Princeton NJ, 08544, USA.ORCID 0000-0002-3215-4026
H ZhaoDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Q YuLewis-Sigler Institute for Integrative Genomics, Princeton NJ, 08544, USA.ORCID 0000-0003-0610-3484
L W WiesnerDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
J F BotelloDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
A DonlicDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0002-0423-618X
E SoehalimOmenn-Darling Bioengineering Institute, Princeton University, Princeton NJ, 08544, USA.
C ZorbasRNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, Belgium.ORCID 0000-0002-1861-3644
L WacheulRNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, Belgium.
A KošmrljDepartment of Mechanical and Aerospace Engineering, Princeton NJ, 08544, USA.ORCID 0000-0001-6137-9200
Dlj LafontaineRNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, Belgium.ORCID 0000-0001-7295-6288
S KlingeLaboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY 10065, USA.ORCID 0000-0002-9373-4737
C P BrangwynneDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.

Funding

Assembly of the eukaryotic small ribosomal subunitR01GM145950 · NIGMS · ROCKEFELLER UNIVERSITY · PI KLINGE, SEBASTIAN · 2022 to 2024
$1.0M
Assembly of the eukaryotic large ribosomal subunitR01GM143181 · NIGMS · ROCKEFELLER UNIVERSITY · PI KLINGE, SEBASTIAN · 2022 to 2023
$678k
NIGMS NIH HHS R01 GM143181NIGMS NIH HHS R01 GM145950
6 · The paper itself

Abstract

Biomolecular condensates are key features of intracellular compartmentalization. As the most prominent nuclear condensate in eukaryotes, the nucleolus is a layered multiphase liquid-like structure and the site of ribosome biogenesis. In the nucleolus, ribosomal RNAs (rRNAs) are transcribed and processed, undergoing multiple maturation steps that ultimately result in formation of the ribosomal small subunit (SSU) and large subunit (LSU). However, how rRNA processing is coupled to the layered nucleolar organization is poorly understood due to a lack of tools to precisely monitor and perturb nucleolar rRNA processing dynamics. Here, we developed two complementary approaches to spatiotemporally map rRNA processing and engineer

Identifiers

PMID39386460
PMCPMC11463421

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.