Evidence map›Paper›PMID 39386427›Full record

ArticlebioRxiv : the preprint server for biology2024

Cholinergic Neuronal Activity Promotes Diffuse Midline Glioma Growth through Muscarinic Signaling.

Richard Drexler, Antonia Drinnenberg, Avishai Gavish, Belgin Yalcin, Kiarash Shamardani, Abigail Rogers, Rebecca Mancusi, Kathryn R Taylor, Yoon Seok Kim, Pamelyn J Woo and 12 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

Richard DrexlerDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-4830-3671
Antonia DrinnenbergDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Avishai GavishDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Belgin YalcinDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0001-9689-9062
Kiarash ShamardaniDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-2548-705X
Abigail RogersDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0003-4889-6566
Rebecca MancusiDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Kathryn R TaylorDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Yoon Seok KimDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Pamelyn J WooDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0009-0008-7509-8061
Alexandre RavelDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Eva TatlockDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.
Charu RamakrishnanDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-3474-6332
Alberto E Ayala-SarmientoBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
David Rincon Fernandez PachecoBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
La'Akea SivertsAllen Institute for Brain Science, Seattle, WA, USA.
Tanya L DaigleAllen Institute for Brain Science, Seattle, WA, USA.ORCID 0000-0001-9700-8452
Bosiljka TasicAllen Institute for Brain Science, Seattle, WA, USA.ORCID 0000-0002-6861-4506
Hongkui ZengAllen Institute for Brain Science, Seattle, WA, USA.ORCID 0000-0002-0326-5878
Joshua J BreunigBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.ORCID 0000-0002-3735-3390
Karl DeisserothDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0001-9440-3967
Michelle MonjeDepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0002-3547-237X

Funding

Targeting the Vasular SystemP50CA165962 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DAPHNE A. HAAS-KOGAN · 2013 to 2026
$33.5M
eDyNAmiC - STANFORDOT2CA278688 · NCI · STANFORD UNIVERSITY · PI PAUL S MISCHEL · 2022 to 2026
$7.7M
Targeting the neuronal microenvironment in glioblastomaU19CA264504 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI Michelle Monje-Deisseroth · 2021 to 2026
$6.4M
Glioma Circuitry: Bridging Systems Neuroscience and CancerDP1NS111132 · NINDS · STANFORD UNIVERSITY · PI MONJE-DEISSEROTH, MICHELLE · 2018 to 2022
$5.7M
Neuronal Regulation of Low-Grade GliomagenesisR01CA258384 · NCI · STANFORD UNIVERSITY · PI David H Gutmann, Michelle Monje-Deisseroth · 2022 to 2026
$3.1M
Neuronal activity-regulated mechanisms of glioma growthR01NS092597 · NINDS · STANFORD UNIVERSITY · PI MONJE-DEISSEROTH, MICHELLE · 2016 to 2021
$2.1M
GESTALT Barcoding and Single-cell Transcriptomics of Tumor Cell Evolution in Personalized Tumor ModelsR33CA236687 · NCI · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2019 to 2021
$1.2M
NCI NIH HHS OT2 CA278688NCI NIH HHS P50 CA165962NCI NIH HHS R01 CA258384NCI NIH HHS R33 CA236687NCI NIH HHS U19 CA264504NINDS NIH HHS DP1 NS111132NINDS NIH HHS R01 NS092597
6 · The paper itself

Abstract

Neuronal activity promotes the proliferation of healthy oligodendrocyte precursor cells (OPC) and their malignant counterparts, gliomas. Many gliomas arise from and closely resemble oligodendroglial lineage precursors, including diffuse midline glioma (DMG), a cancer affecting midline structures such as the thalamus, brainstem and spinal cord. In DMG, glutamatergic and GABAergic neuronal activity promotes progression through both paracrine signaling and through bona-fide neuron-to-glioma synapses. However, the putative roles of other neuronal subpopulations - especially neuromodulatory neurons located in the brainstem that project to long-range target sites in midline anatomical locations where DMGs arise - remain largely unexplored. Here, we demonstrate that the activity of cholinergic midbrain neurons modulates both healthy OPC and malignant DMG proliferation in a circuit-specific manner at sites of long-range cholinergic projections. Optogenetic stimulation of the cholinergic pedunculopontine nucleus (PPN) promotes glioma growth in pons, while stimulation of the laterodorsal tegmentum nucleus (LDT) facilitates proliferation in thalamus, consistent with the predominant projection patterns of each cholinergic midbrain nucleus. Reciprocal signaling was evident, as increased activity of cholinergic neurons in the PPN and LDT was observed in pontine DMG-bearing mice. In co-culture, hiPSC-derived cholinergic neurons form neuron-to-glioma networks with DMG cells and robustly promote proliferation. Single-cell RNA sequencing analyses revealed prominent expression of the muscarinic receptor genes

Identifiers

PMID39386427
PMCPMC11463519

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.