Evidence map›Paper›PMID 39386424›Full record

ArticlebioRxiv : the preprint server for biology2025

Galvanin (TMEM154) is an electric-field sensor for directed cell migration.

Nathan M Belliveau, Matthew J Footer, Amy Platenkamp, Celeste Rodriguez, Heonsu Kim, Christopher K Prinz, Aaron P van Loon, Yubin Lin, Tara E Eustis, Michelle M Chan and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Nathan M BelliveauDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-1536-1963
Matthew J FooterDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-3365-2916
Amy PlatenkampDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0001-9251-7407
Celeste RodriguezDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0009-0000-4399-7742
Heonsu KimDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0001-5703-7793
Christopher K PrinzDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0003-1535-6707
Aaron P van LoonDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-8597-1572
Yubin LinDepartment of Electrical and Computer Engineering, Princeton University, Princeton, NJ 08540, USA.ORCID 0000-0003-4476-9383
Tara E EustisDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0003-4448-7084
Michelle M ChanDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0001-9451-9716
Daniel J CohenDepartment of Mechanical and Aerospace Engineering and Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ 08540, USA.ORCID 0000-0001-5819-1135
Julie A TheriotDepartment of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-2334-2535

Funding

A Functional Genomics Approach to Uncover the Mechanisms of Neutrophil Galvanotaxis.R00GM147355 · NIGMS · UNIVERSITY OF COLORADO · PI Nathan M Belliveau · 2025 to 2026
$498k
A Functional Genomics Approach to Uncover the Mechanisms of Neutrophil Galvanotaxis.K99GM147355 · NIGMS · UNIVERSITY OF WASHINGTON · PI BELLIVEAU, NATHAN M · 2022 to 2023
$225k
NIGMS NIH HHS K99 GM147355NIGMS NIH HHS R00 GM147355
6 · The paper itself

Abstract

Directed cell migration of immune and epithelial cells is critical for their rapid response to tissue injury or infection. Endogenous electric fields generated by disruption of the transepithelial potential across the skin have been postulated to play an important role in guiding cells to wound sites, though how individual cells sense these tissue-scale physical cues remains largely unknown. We have identified Galvanin (TMEM154), a previously uncharacterized single-pass transmembrane protein, as being required for electric-field-guided migration of individual rapidly moving cells. Galvanin functions in both immune and epithelial cell types. Upon exposure of cells to an electric field, Galvanin rapidly relocalizes to the anodal side of a cell, and the net charge on its extracellular domain is necessary and sufficient to drive this spatial relocalization. Furthermore, expression of Galvanin is sufficient to confer electric field-guided migration on otherwise non-responsive epithelial cells. In human neutrophils, we show that Galvanin relocalization is immediately followed by changes in the spatial pattern of cellular protrusion and retraction. The strong directional response of these cells is lost upon truncation of Galvanin's intracellular domain, suggesting that Galvanin acts as a direct sensor of the electric field, transducing spatial information about a cell's electrical environment to the intracellular migratory apparatus. This sensor relocalization mechanism of cell steering defines a new paradigm for directed cell migration.

Identifiers

PMID39386424
PMCPMC11463530

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.