ArticlePNAS nexus2024
High accuracy meets high throughput for near full-length 16S ribosomal RNA amplicon sequencing on the Nanopore platform.
Article in PNAS nexus, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Synthetic microbial communities mediate maize growth and soil microecology under saline-alkali stress.World journal of microbiology & biotechnology · 2026Article
- A scalable and cost-effective nanopore workflow for 16S rRNA microbiome profiling validated in a zebrafish gut model.Microbial genomics · 2026Article
- Phylogenetic inference under the balanced minimum evolution criterion via semidefinite programming.Bioinformatics (Oxford, England) · 2026Article
- Article
- From microbiome profiling to precision medicine: diagnostic and therapeutic potential in gastrointestinal disorders: current evidence, challenges, and future directions.Annals of medicine and surgery (2012) · 2026Review
- Oxford Nanopore enhanced accuracy of long-read amplicons applied to microbial whole-genome sequencing.Microbiology spectrum · 2026Article
- Preliminary microbiome characterization of shrimp gut and pond water in Egyptian aquaculture farms: Implications for pathogen dynamics and management practices.Veterinary research communications · 2026Article
- RUMINA: high-throughput deduplication of unique molecular identifiers for amplicon and whole-genome sequencing with enhanced error correction.Bioinformatics (Oxford, England) · 2026Article
- CoSMIC: A hybrid approach for large-scale, high-resolution microbial profiling of novel niches.PloS one · 2026Article
- Effect of sialidase inhibitors on a plaque community biofilm model.Access microbiology · 2026Article
- Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa.Scientific data · 2025Article
- Automated environmental metagenomics using Oxford nanopore sequencing.BMC genomics · 2025Article
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- Impact of DNA extraction techniques and sequencing approaches on microbial community profiling accuracy.Frontiers in microbiomes · 2025Article
- What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in DecayingJournal of fungi (Basel, Switzerland) · 2024Article
- GROND: a quality-checked and publicly available database of full-length 16S-ITS-23S rRNA operon sequences.Microbial genomics · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small subunit (SSU) ribosomal RNA (rRNA) gene amplicon sequencing is a foundational method in microbial ecology. Currently, short-read platforms are commonly employed for high-throughput applications of SSU rRNA amplicon sequencing, but at the cost of poor taxonomic classification due to limited fragment lengths. The Oxford Nanopore Technologies (ONT) platform can sequence full-length SSU rRNA genes, but its lower raw-read accuracy has so-far limited accurate taxonomic classification and de novo feature generation. Here, we present a sequencing workflow, termed
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