Evidence map›Paper›PMID 39385089›Full record

ArticleBMC plant biology2024

Genome-wide analysis of tobacco NtTOM1/TOM3 gene family and identification of NtTOM1a/ NtTOM3a response to tobacco mosaic virus.

Xuebo Wang, Yalin Bai, Zhan Shen, Xinyao Zhang, Changchun Cai, Chan Qiao, Caihong Jiang, Lirui Cheng, Dan Liu, Aiguo Yang

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Genome-Wide Identification and Expression Analysis of theCurrent issues in molecular biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xuebo Wang *Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Yalin Bai *Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Zhan ShenKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Xinyao ZhangTechnology Center, China Tobacco Hunan Industrial Co., Ltd, Changsha, 410007, China.
Changchun CaiTobacco Research Institute of Hubei Province, Wuhan, 430030, China.
Chan QiaoTobacco Research Institute of Mudanjiang, Harbin, 150076, China.
Caihong JiangKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Lirui ChengKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Dan LiuKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. liudan@caas.cn.
Aiguo YangKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, China. yangaiguo@caas.cn.

Funding

Agricultural Science and Technology Innovation Program of CAAS ASTIP-TRIC01the China Tobacco Hunan Industrial Co., Ltd. Research Project KY2019YC0003The key Science and Technology Program of Heilongjiang Tobacco Corporation 2022230000200047The key Science and Technology Program of Hubei Tobacco Corporation 027Y2020-014Tobacco Genome Project of China National Tobacco Corporation 110202001019(JY-02)
6 · The paper itself

Abstract

backgroundTOBAMOVIRUS MULTIPLICATION 1 (TOM1) and its homolog TOBAMOVIRUS MULTIPLICATION 3 (TOM3) play a prominent role in the multiplication of tobacco mosaic virus (TMV) in higher plants. Although homologs of NtTOM1/TOM3 genes have been identified in several plant species, little is known about the characteristics and functions of NtTOM1/TOM3 at the genome-wide level in tobacco (Nicotiana tabacum L.).

resultsIn this study, we performed genome-wide identification and expression pattern analysis of the tobacco NtTOM1/TOM3 gene family. Twelve NtTOM1/TOM3 genes were identified and classified into four groups based on phylogenetic analysis. Sequence and conserved domain analyses showed that all these genes contained a specific DUF1084 domain. Expression pattern analysis showed that NtTOM1a, NtTOM1b, NtTOM1d, NtTOM3a, NtTOM3b, and NtTOM3d were induced by TMV at 1-, 3-, and 9 dpi, whereas the expression of other genes was not responsive to TMV at the early infection stage. TMV virion accumulation showed no obvious difference in either nttom1a or nttom3a mutants compared with the wild type. However, the virus propagation was significantly, but not completely, inhibited in the nttom1atom3a double mutant, indicating that other gene family members may function redundantly, such as NtTOM1b and NtTOM1d. In addition, overexpression of NtTOM1a or NtTOM3a also inhibited the TMV replication to some extent.

conclusionsThe present study performed genome-wide analysis of the NtTOM1/TOM3 gene family in tobacco, and identified NtTOM1a and NtTOM3a as important genes involved in TMV multiplication based on functional analysis. These results provide a theoretical basis for further improving TMV resistance in tobacco.

Indexed as

Multigene FamilyNicotianaPhylogenyPlant ProteinsTobacco Mosaic VirusGene Expression Regulation, PlantGenes, PlantGenome, PlantGenome-Wide Association StudyPlant DiseasesPlant ProteinsTobaccoTobacco mosaic virusTobamovirus multiplication proteinTOM1TOM3

Identifiers

PMID39385089
PMCPMC11465672

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.