ArticleNature biotechnology2025
One-step nanoscale expansion microscopy reveals individual protein shapes.
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Article in Nature biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed.
- Nanorulers for Super-Resolution Microscopy.Chembiochem : a European journal of chemical biology · 2026Review
- Hydrogel Chemistry in Expansion Microscopy: From Polyacrylamide to Next-Generation Swellable Polymers.Chembiochem : a European journal of chemical biology · 2026Review
- Resolving endogenous protein organization in cells with nanometer resolution.Nature communications · 2026Article
- Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins.Chemical & biomedical imaging · 2026Article
- Thousandfold Expansion Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Artificial Intelligence for Discovery in Life Sciences.Bioconjugate chemistry · 2026Review
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- IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.PLoS biology · 2026Article
- Large-scale visualization of α-synuclein oligomers in Parkinson's disease brain tissue.Nature biomedical engineering · 2026Article
- Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples.Nature protocols · 2026Review
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- The Fantastic Single-Molecule Techniques.Chemical & biomedical imaging · 2026Review
- Functional super-resolution microscopy of fibers and polymers: convergence of artificial and biological systems at the nanoscale.Nanoscale horizons · 2026Review
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Tensile expansion microscopy applies mechanical force to super-resolve fixed and image live cellular samples.bioRxiv : the preprint server for biology · 2026Article
- T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies.Journal of extracellular vesicles · 2026Article
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Principles ofbioRxiv : the preprint server for biology · 2026Article
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Authors and funding
38 authors.
Ali H ShaibInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany. ali.shaib@med.uni-goettingen.de.
Abed Alrahman ChouaibDepartment of Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Homburg, Germany.
Rajdeep ChowdhuryInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Jonas AltendorfInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0009-0003-2515-8330
Daniel MihaylovMRC Laboratory of Molecular Biology, Cambridge, UK.
Chi ZhangDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.
Donatus KrahInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Vanessa ImaniInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Russell K W SpencerInstitute for Theoretical Physics, Georg-August University, Göttingen, Germany.ORCID http://orcid.org/0000-0002-4814-9010
Svilen Veselinov GeorgievInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Nikolaos MougiosInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Mehar MongaBiochemistry of Membrane Dynamics Group, Institute for Auditory Neuroscience, University Medical Center Göttingen, Göttingen, Germany.
Sofiia ReshetniakInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0000-0003-4847-4144
Tiago MimosoInstitute for X-Ray Physics, University of Göttingen, Göttingen, Germany.
Han ChenInstitute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0009-0003-1357-5161
Parisa FatehbasharzadInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Dagmar CrzanInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Kim-Ann SaalInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Mohamad Mahdi AlawiehInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.
Nadia AlawarDepartment of Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Homburg, Germany.ORCID http://orcid.org/0000-0002-8591-2513
Janna EiltsDepartment of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, Würzburg, Germany.
Jinyoung KangDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.
Alireza SoleimaniInstitute for Theoretical Physics, Georg-August University, Göttingen, Germany.
Marcus MüllerInstitute for Theoretical Physics, Georg-August University, Göttingen, Germany.ORCID http://orcid.org/0000-0002-7472-973X
Constantin PapeInstitute of Computer Science, Georg-August University Göttingen, Göttingen, Germany.ORCID http://orcid.org/0000-0001-6562-7187
Luis AlvarezLeica Microsystems CMS GmbH, Mannheim, Germany.ORCID http://orcid.org/0000-0003-1316-1906
Claudia TrenkwalderDepartment of Neurosurgery, University Medical Center, Göttingen, Germany.
Brit MollenhauerParacelsus-Elena-Klinik, Kassel, Germany.
Tiago F OuteiroDepartment of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0000-0003-1679-1727
Sarah KösterInstitute for X-Ray Physics, University of Göttingen, Göttingen, Germany.
Julia PreobraschenskiBiochemistry of Membrane Dynamics Group, Institute for Auditory Neuroscience, University Medical Center Göttingen, Göttingen, Germany.
Ute BechererDepartment of Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Homburg, Germany.
Tobias MoserBiochemistry of Membrane Dynamics Group, Institute for Auditory Neuroscience, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0000-0001-7145-0533
Edward S BoydenDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-0419-3351
A Radu AricescuMRC Laboratory of Molecular Biology, Cambridge, UK.ORCID http://orcid.org/0000-0003-3783-1388
Markus SauerDepartment of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, Würzburg, Germany.ORCID http://orcid.org/0000-0002-1692-3219
Felipe OpazoInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.ORCID http://orcid.org/0000-0002-4968-9713
Silvio O RizzoliInstitute for Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany. srizzol@gwdg.de.ORCID http://orcid.org/0000-0002-1667-7839
Funding
Expansion MicroscopyR01EB024261 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2025
$4.6MMechanisms of pathology and neuronal hyperactivity in a memory circuit in Alzheimer's diseaseR01AG070831 · NIA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., TSAI, LI-HUEI · 2021 to 2025
$3.2MDeutsche Forschungsgemeinschaft (German Research Foundation) SFB1286/A03/B02/C06/Z04Deutsche Forschungsgemeinschaft (German Research Foundation) SFB894/A9Medical Research Council MC_EX_MR/T046279/1Medical Research Council MC_UP_1201/15Medical Research Council MR/L009609/1NIA NIH HHS R01 AG070831NIBIB NIH HHS R01 EB024261RCUK | Medical Research Council (MRC) MC_EX_MR/T046279/1RCUK | Medical Research Council (MRC) MC_UP_1201/15RCUK | Medical Research Council (MRC) MR/L009609/1RCUK | MRC | Medical Research Foundation MR/L009609/1
Abstract
The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold, label them with conventional fluorophores and image them with conventional light microscopes, acquiring videos in which we analyze fluorescence fluctuations. One-step nanoscale expansion (ONE) microscopy enables the visualization of the shapes of individual membrane and soluble proteins, achieving around 1-nm resolution. We show that conformational changes are readily observable, such as those undergone by the ~17-kDa protein calmodulin upon Ca
Indexed as
NanotechnologyProteinsCalciumCalmodulinHumansMicroscopy, FluorescenceParkinson DiseaseProtein ConformationCalciumCalmodulinProteins
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