ArticleLight, science & applications2024
Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.
Article in Light, science & applications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- NIR-II squeezed light-field microscopy enables high-speed volumetric imaging of deep-tissue dynamicsbioRxiv : the preprint server for biology · 2026Article
- Wide-field two-photon random illumination microscopy (2P-RIM).Science advances · 2026Article
- Goblet cells mechanically breach the epithelial barrier in gut homeostasis.Nature communications · 2026Article
- Single-shot extended-depth-of-field fourier light field microscopy enabled by a distorted grating.Biomedical optics express · 2026Article
- Implementation and Optimization of a Random Illumination Microscope: towards Robustness for Microscopy Core Facility.Biology of the cell · 2026Article
- Random Illumination Microscopy: faster, thicker, and aberration-insensitive.Light, science & applications · 2025Article
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Authors and funding
15 authors.
Funding
Abstract
The ultimate aim of fluorescence microscopy is to achieve high-resolution imaging of increasingly larger biological samples. Extended depth of field presents a potential solution to accelerate imaging of large samples when compression of information along the optical axis is not detrimental to the interpretation of images. We have implemented an extended depth of field (EDF) approach in a random illumination microscope (RIM). RIM uses multiple speckled illuminations and variance data processing to double the resolution. It is particularly adapted to the imaging of thick samples as it does not require the knowledge of illumination patterns. We demonstrate highly-resolved projective images of biological tissues and cells. Compared to a sequential scan of the imaged volume with conventional 2D-RIM, EDF-RIM allows an order of magnitude improvement in speed and light dose reduction, with comparable resolution. As the axial information is lost in an EDF modality, we propose a method to retrieve the sample topography for samples that are organized in cell sheets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.