Evidence map›Paper›PMID 39382591›Full record

ArticleArchives of microbiology2024

Insights into the methodological perspectives for screening polyunsaturated fatty acids-containing bacteria.

Vishnu Ramachandran, Sumithra Thangalazhy Gopakumar, Krupesha Sharma Sulumane Ramachandra, S Chandrasekar, C S Tejpal, Anusree Velappan Nair, Sayooj Pootholathil, K R Sreenath, J K Nithyashree, Gopalakrishnan Achamveetil

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Vishnu RamachandranMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
Sumithra Thangalazhy GopakumarMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India. sumithravet@gmail.com.ORCID http://orcid.org/0000-0001-9794-6772
Krupesha Sharma Sulumane RamachandraMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
S ChandrasekarMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
C S TejpalBiochemistry and Nutrition Division, ICAR-CIFT, Willingdon Island Matsyapuri P.O., Cochin, Kerala, 682029, India.
Anusree Velappan NairMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
Sayooj PootholathilMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
K R SreenathMarine Diversity and Environment Division, ICAR-CMFRI, Post Box No. 1603, Kochi, 682018, India.
J K NithyashreeMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.
Gopalakrishnan AchamveetilMarine Biotechnology, Fish Nutrition, and Health Division, ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI), Post Box No. 1603, Ernakulam North P.O., Kochi, 682018, India.

Funding

CSIR-UGC For PhDDepartment of Biotechnology, Ministry of Science and Technology, India BT/AAQ/3/SP28267/2018
6 · The paper itself

Abstract

Polyunsaturated fatty acids (PUFA) are vital molecules in the pharmaceutical, medical, and nutritional industries. Exploration of bacterial strains capable of producing significant amounts of PUFAs offers a promising avenue for biotechnological applications and industrial-scale production. However, an extensive screening of several samples from diverse sources is highly needed to identify a potential strain. The present study provides the results of the evaluation of 15 different screening methodologies (including changes in existing protocols in terms of reagent concentration, incubation temperature and time) for identifying PUFA-producing bacteria in comparison to the gold standard method (Gas chromatography-mass spectrometry), for the first time. The results determined the most effective techniques for each critical PUFA, leading to an optimized screening process that saves time and resources. The H

Indexed as

BacteriaFatty Acids, UnsaturatedGas Chromatography-Mass SpectrometryHydrogen PeroxideFatty Acids, UnsaturatedHydrogen PeroxideDocosahexaenoic acidEicosapentaenoic acidPUFA biosynthetic genesSemiquantitative screening methodology

Identifiers

PMID39382591

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.