Evidence map›Paper›PMID 39379991›Full record

ArticleProteome science2024

NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status.

Andressa Peres de Oliveira, Claudia D C Navarro, Pedro Rafael F Dias, Tania Arguello, Brittni R Walker, Sandra R Bacman, Lizandra Maia Sousa, Roger F Castilho, Sílvio R Consonni, Carlos T Moraes and 1 more

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In one paragraph

Article in Proteome science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Andressa Peres de OliveiraFaculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas, Rua Cândido Portinari, 200 Cidade Universitária Zeferino Vaz, Campinas, SP, CEP 13083-871, Brazil.
Claudia D C NavarroDepartamento de Patologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Pedro Rafael F DiasFaculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas, Rua Cândido Portinari, 200 Cidade Universitária Zeferino Vaz, Campinas, SP, CEP 13083-871, Brazil.
Tania ArguelloDepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL, USA.
Brittni R WalkerDepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL, USA.
Sandra R BacmanDepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL, USA.
Lizandra Maia SousaDepartamento de Bioquímica E Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Roger F CastilhoDepartamento de Patologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Sílvio R ConsonniDepartamento de Bioquímica E Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Carlos T MoraesDepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL, USA. cmoraes@med.miami.edu.
Jörg KobargFaculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas, Rua Cândido Portinari, 200 Cidade Universitária Zeferino Vaz, Campinas, SP, CEP 13083-871, Brazil. jorgkoba@unicamp.br.

Funding

SETTING THE STAGE FOR REPLACEMENT OF MITOCHONDRIAL GENESR01EY010804 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI MORAES, CARLOS TORRES · 1995 to 2025
$9.4M
Cellular and Molecular Consequences of Respiratory Chain Defects in NeuronsR01NS079965 · NINDS · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI MORAES, CARLOS TORRES · 2012 to 2023
$3.9M
Fapesp 2022/15126-9NEI NIH HHS R01 EY010804NINDS NIH HHS R01 NS079965
6 · The paper itself

Abstract

backgroundNEK10, a serine/threonine/tyrosine kinase belonging to the NEK (NIMA-related kinases) family, has been associated with diverse cellular processes. However, no specific target pathways have been identified. Our previous work knocking down NEK10 in HeLa cells suggested a functional association with mitochondria, as we observed altered mitochondrial morphology, mitochondrial oxygen consumption, mtDNA integrity, and reactive oxygen species levels.

methodsTo better understand this association, we studied human HAP1 cells fully knockout for NEK10 and confirmed that NEK10 has an important role in mitochondrial homeostasis. We performed the study of mitochondrial respiration, mitochondrial morphology, mitochondrial mass, and mtDNA analysis. Additionally, we showed proteome and phosphoproteome data of crude mitochondrial fraction of Parental and NEK10 KO cells using liquid chromatography-mass spectrometry (LC-MS/MS).

resultsIn the absence of NEK10 several mitochondrial functions were disturbed. Moreover, proteome and phosphoproteome analyses of mitochondrial fractions showed that NEK10 alters the threonine phosphorylation status of several mitochondrial/endoplasmic reticulum components, including HSP60, NDUFB4, and TOM20. These changes impacted the steady-state levels of a larger group of proteins, preferentially involving respiratory complexes and autophagy pathways.

conclusionWe concluded that NEK10 plays a key role in mitochondrial function, possibly by modulating the phosphorylation status of mitochondrial proteins.

Identifiers

PMID39379991
PMCPMC11460017

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.