Evidence map›Paper›PMID 39379812›Full record

ArticleBMC microbiology2024

Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR.

Abebe Fromsa, Andrew J K Conlan, Sreenidhi Srinivasan, Miserach Zeleke, Dawit Worku, Matios Lakew, Musse Girma Abdela, Getahun Bahiru, James L N Wood, Douwe Bakker and 3 more

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Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Bovine tuberculosis reactor cattle in Southwest Ethiopia: Risk factors for bovine tuberculosis.Journal of clinical tuberculosis and other mycobacterial diseases · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Abebe FromsaAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. abebe.fromsa@aau.edu.et.
Andrew J K ConlanDisease Dynamics Unit, Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. ajkc2@cam.ac.uk.
Sreenidhi SrinivasanHuck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, USA.
Miserach ZelekeAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Dawit WorkuAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Matios LakewAnimal Health Institute, Sebeta, Ethiopia.
Musse Girma AbdelaAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Getahun BahiruMinistry of Agriculture, Addis Ababa, Ethiopia.
James L N WoodDisease Dynamics Unit, Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Douwe BakkerIndepedent Researcher, Lelystad, The Netherlands.
Balako GumiAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Gobena AmeniAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. gobena.ameni@uaeu.ac.ae.
Vivek KapurHuck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, USA. vkapur@psu.edu.

Funding

This research was funded by the Bill & Melinda Gates Foundation under the project "Accelerating Bovine Tuberculosis Control in Developing Countries (AB TB CD)" OPP1176950
6 · The paper itself

Abstract

backgroundBovine tuberculosis (bTB) is a chronic disease caused by members of the Mycobacterium tuberculosis complex (MTBC) that ultimately leads to the development of progressive granulomatous lesions. Although the disease is widespread, especially in crossbred cattle in Ethiopia, routine investigations and surveillance are lacking. Thus, the aim of this study was to determine the prevalence, associated risk factors, and species of mycobacteria causing bTB in slaughtered cattle at four slaughterhouses in Central Ethiopia.

methodsPostmortem examination of 7,640 cattle was conducted using a cross-sectional slaughterhouse survey. A total of 388 tuberculous-like lesions (TBLs) were collected from 173 animals and cultured. Six target genes were used to differentiate mycobacterial species using multiplex real-time PCR (mRT-PCR). Multivariate logistic regression analyses and related odds ratios (ORs) were used to gauge the strength of the associations between risk factors, TBL incidence and culture growth.

resultsThe prevalence of TBL was 2.3% (95% CI = 2.0-2.6). Logistic regression analysis indicated an increased risk of TBL in crossbred cattle (OR = 11.8, 95% CI: 6.4, 21.2, p < 0.001). Animals slaughtered at Adama (OR = 3.2, 95% CI: 1.2, 7.3, p = 0.009) or Burayu (OR = 5.8, 95% CI: 3.9, 8.9, p < 0.001) had a greater risk of TBL than those slaughtered at Sululta. There were significantly more TBL-positive lesions in the lungs and lymph nodes related to the lung (OR = 7.1; 95% CI: 2.7, 24.5, p < 0.001) and the head lymph node (OR = 5.6; 95% CI: 1.8, 21.7; p = 0.006) compared to gut associated lymph nodes. Among the 173 TBL-positive animals, 36% (95% CI = 28.8, 43.2), and among the 388 TBL-positive tissues, 24.2% (95% CI = 20, 29) were culture and mRT-PCR positive. All the culture-generated isolates were positive for M. bovis in mRT-PCR. Among them, two animals had mixed infections including one zebu cattle tested positive for both M. caprae and M. bovis, and a crossbred cow tested positive for both M. tuberculosis and M. bovis in mRT-PCR. This suggests persistent transmission within the cattle population, posing a substantial public health threat.

conclusionThis study revealed an eleven-fold greater risk of bTB-related lesions in crossbred cattle compared to local zebu cattle. This finding highlights the necessity for targeted interventions, continuous vigilance, and thorough carcass inspection to mitigate public health risks.

Indexed as

AbattoirsMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionTuberculosis, BovineAnimalsCattleCross-Sectional StudiesEthiopiaFemaleMycobacterium bovisPrevalenceRisk FactorsBovine tuberculosisCattleMycobacterium tuberculosis complexSlaughterhouseTuberculous-like lesions

Identifiers

PMID39379812
PMCPMC11462752

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.