Evidence map›Paper›PMID 39379735›Full record

ArticleMikrochimica acta2024

Magnetic microbead-based upconversion immunoassay with laser-induced breakdown spectroscopy readout for the detection of prostate-specific antigen.

Eva Zikmundová, Dorota Sklenárová, Eva Kočí, Terezie Zatloukalová, Tereza Bačová, Ekaterina Makhneva, Daniel Holub, Eliška Macháčová, Hana Kopřivová, Karolína Vytisková and 5 more

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Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Eva Zikmundová *Central European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Dorota Sklenárová *Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Eva KočíCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Terezie ZatloukalováDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Tereza BačováFaculty of Mechanical Engineering (FME), Brno University of Technology, Technická 2896, 616 69, Brno, Czech Republic.
Ekaterina MakhnevaDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Daniel HolubCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Eliška MacháčováDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Hana KopřivováCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Karolína VytiskováCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Pavel PořízkaCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Karel NovotnýDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Petr SkládalDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Zdeněk FarkaDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. farka@mail.muni.cz.ORCID http://orcid.org/0000-0002-6842-7081
Jozef KaiserCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.

Funding

Brno City Municipality Brno Ph.D. Talent ScholarshipBrno University of Technology CEITEC VUT-J-23-8207Brno University of Technology FSI-S-23-8389Grantová Agentura České Republiky 22-27580S
6 · The paper itself

Abstract

Laser-induced breakdown spectroscopy (LIBS) is a promising technique for the readout of immunochemical assays utilizing indirect detection of labels (Tag-LIBS), typically based on nanoparticles. We have previously demonstrated that Tag-LIBS immunoassay employing yttrium-based photon-upconversion nanoparticles (UCNPs) can reach sensitivity similar to commonly used enzyme and fluorescence immunoassays. In this study, we report on further increasing the sensitivity of UCNP-based Tag-LIBS immunoassay by employing magnetic microbeads (MBs) as the solid phase in the determination of cancer biomarker prostate-specific antigen. Due to the possibility of analyte preconcentration, MBs enabled achieving a limit of detection (LOD) of 4.0 pg·mL

Indexed as

LasersLimit of DetectionProstate-Specific AntigenHumansImmunoassayMaleMicrospheresSpectrum AnalysisYttriumProstate-Specific AntigenYttriumDouble-pulse LIBSMagnetic microparticlePhoton-upconversion nanoparticleProstate-specific antigenTag-LIBS

Identifiers

PMID39379735

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.