Evidence map›Paper›PMID 39377838›Full record

ArticleApplied microbiology and biotechnology2024

Identifying sesterterpenoids via feature-based molecular networking and small-scale fermentation.

Kangjie Lv, Yuyang Duan, Xiaoying Li, Xinye Wang, Cuiping Xing, Keying Lan, Bin Zhu, Guoliang Zhu, Yuyang Qiu, Songwei Li and 4 more

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Kangjie Lv *State Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Yuyang Duan *State Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Xiaoying LiState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Xinye WangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Cuiping XingState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Keying LanState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Bin ZhuLab of Pharmaceutical Crystal Engineering Research and Technology, East China University of Science and Technology, Shanghai, 200237, China.
Guoliang ZhuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Yuyang QiuSchool of Insurance, Shandong University of Finance and Economics, Jinan, 250014, China.
Songwei LiSchool of Medicine, Shanghai University, Shanghai, 200444, China.
Tom HsiangSchool of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Lixin ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China.
Lan JiangDepartment of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210093, China. jianglan0426@163.com.
Xueting LiuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, 200237, China. liuxueting@ecust.edu.cn.ORCID http://orcid.org/0000-0002-1322-8253

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Terpenoids are known for their diverse structures and broad bioactivities with significant potential in pharmaceutical applications. However, natural products with low yields are usually ignored in traditional chemical analysis. Feature-based molecular networking (FBMN) was developed recently to cluster compounds with similar skeletons, which can highlight trace amounts of unknown compounds. Fusoxypene A is a sesterterpene synthesized by Fusarium oxysporum fusoxypene synthase (FoFS) with a unique 5/6/7/3/5 ring system. In this study, the FoFS-containing biosynthetic gene cluster was identified from F. oxysporum FO14005, and an efficient FBMN-based strategy was established to characterize four new sesterterpenoids, fusoxyordienoid A-D (1-4), based on a small-scale fermentation strategy. A cytochrome P450 monooxygenase, FusB, was found to be involved in the functionalization of fusoxypene A at C-17 and C-24 and responsible for the hydroxylation of fusoxyordienoid A at C-1 and C-8. This study highlights the potential of FBMN as a powerful tool for the discovery and characterization of natural compounds with low abundance. KEY POINTS: Combined small-scale fermentation and FBMN for rapid discovery of fusoxyordienoids Characterization of four new fusoxyordienoids with 5/6/7/3/5 ring system Biosynthetic pathway elucidation via tandem expression and substrate feeding.

Indexed as

FermentationFusariumMultigene FamilySesterterpenesBiological ProductsBiosynthetic PathwaysCytochrome P-450 Enzyme SystemBiological ProductsCytochrome P-450 Enzyme SystemSesterterpenesAspergillus oryzaeBifunctional terpene synthasesCytochrome P450Feature-based molecular networkingHeterologous expression

Identifiers

PMID39377838
PMCPMC11461746

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.