Evidence map›Paper›PMID 39373150›Full record

ArticleBiology open2024

Endonuclease G is dispensable for sperm mitochondrial DNA elimination during spermatogenesis in mice.

Xuefeng Xie, Jianshuang Li, Xue Zhang, Shaomei Mo, Ang Li, Tian-Yi Sun, Feng-Yun Xie, Shi-Ming Luo, Guang Wang, Xiang-Hong Ou and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xuefeng XieGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Jianshuang LiDepartment of Orthopaedics, Guangzhou Red Cross Hospital, Faculty of Medical Science, Jinan University, Guangzhou, 510220, Guangdong, China.
Xue ZhangGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Shaomei MoGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Ang LiGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Tian-Yi SunGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Feng-Yun XieGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Shi-Ming LuoGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Guang WangGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Xiang-Hong OuGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Qing-Yuan SunGuangdong Second Provincial General Hospital, Postdoctoral Research Station of Basic Medicine, School of Medicine, Jinan University, Guangzhou, 510317, Guangdong, China.
Qinghua ZhouDepartment of Orthopaedics, Guangzhou Red Cross Hospital, Faculty of Medical Science, Jinan University, Guangzhou, 510220, Guangdong, China.ORCID 0000-0002-0148-2414

Funding

Guangdong Basic and Applied Basic Research Foundation, China 2023B1515120089Jinan University Affiliated Guangdong Second Provincial General Hospital: Guangdong Second Provincial General Hospital; Jinan University; Jinan University Affiliated Guangzhou Red Cross Hospital: Guangzhou Red Cross HospitalNational Natural Science Foundation of China 32300953Science and Technology Program of Guangzhou, China 2023A03J0258
6 · The paper itself

Abstract

Maternal inheritance of mitochondrial DNA (mtDNA) is a widespread phenomenon in eukaryotes. Our earlier research indicated that sperm mtDNA is removed prior to fertilization in mice, and Endonuclease G (ENDOG) orchestrates the degradation of sperm mitochondria in Caenorhabditis elegans. However, the mechanisms underlying sperm mtDNA disposal in mammals remain poorly understood. To investigate the potential role of ENDOG in sperm mtDNA elimination, we created Endog knockout (Endog-/-) mice. Our findings revealed that Endog-/- mice maintained normal spermatogenesis and fertility. Most strikingly, we detected no substantial discrepancy in sperm mtDNA copy number between Endog-/- and control mice. Furthermore, we noted that sperm mtDNA copy numbers were unchanged in both less motile and motile sperm isolated by Percoll gradient centrifugation from Endog-/- and control mice. Taken together, our results indicate that ENDOG is not essential for spermatogenesis or the elimination of sperm mtDNA in mice.

Indexed as

DNA, MitochondrialEndodeoxyribonucleasesMice, KnockoutSpermatogenesisSpermatozoaAnimalsFertilityMaleMiceMitochondriaDNA, MitochondrialEndodeoxyribonucleasesendonuclease GEndoGMaternal inheritanceMitochondrial DNAMitochondrial DNA eliminationSperm

Identifiers

PMID39373150
PMCPMC11554256

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.