Evidence map›Paper›PMID 39372759›Full record

ArticlebioRxiv : the preprint server for biology2024

pr-independent biogenesis of infectious mature Zika virus particles.

Kimberly A Dowd, Michelle Schroeder, Egan Sanchez, Beniah Brumbaugh, Bryant M Foreman, Katherine E Burgomaster, Wei Shi, Lingshu Wang, Natalie Caputo, David N Gordon and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Kimberly A DowdArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Michelle SchroederArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Egan SanchezArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Beniah BrumbaughResearch Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, Division of Intramural Research, NIAID, NIH; Hamilton, 59840, USA.
Bryant M ForemanArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Katherine E BurgomasterArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Wei ShiVirology Core, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Lingshu WangVirology Core, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Natalie CaputoArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
David N GordonArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Cindi L SchwartzResearch Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, Division of Intramural Research, NIAID, NIH; Hamilton, 59840, USA.
Bryan T HansenResearch Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, Division of Intramural Research, NIAID, NIH; Hamilton, 59840, USA.
Maya AleshnickArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Wing-Pui KongVirology Core, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Kaitlyn M MorabitoViral Pathogenesis Laboratory, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Heather D HickmanViral Immunity and Pathogenesis Unit, Laboratory of Viral Diseases, Division of Intramural Research, NIAID, NIH; Bethesda, 20892, USA.
Barney S GrahamViral Pathogenesis Laboratory, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.
Elizabeth R FischerResearch Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, Division of Intramural Research, NIAID, NIH; Hamilton, 59840, USA.
Theodore C PiersonArbovirus Immunity Section, Vaccine Research Center, NIAID, NIH; Bethesda, 20892, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flavivirus assembly at the endoplasmic reticulum is driven by the structural proteins envelope (E) and premembrane (prM). Here, contrary to the established paradigm for flavivirus assembly, we demonstrate that the biogenesis of flavivirus particles does not require an intact prM nor proteolytic activation. The expression of E preceded by a truncated version of prM (M-E) was sufficient for the formation of non-infectious Zika virus subviral particles and pseudo-infectious reporter virions. Subviral particles encoded by a ZIKV M-E DNA vaccine elicited a neutralizing antibody response that was insensitive to the virion maturation state, a feature of flavivirus humoral immunity shown to correlate with protection. M-E vaccines that uniformly present structural features shared with mature virions offer a higher quality and broadly applicable approach to flavivirus vaccination.

Identifiers

PMID39372759
PMCPMC11452192

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.