Evidence map›Paper›PMID 39368692›Full record

ArticleExperimental eye research2024

Schlemm's canal-selective Tie2/TEK knockdown induces sustained ocular hypertension in adult mice.

Joon Schwakopf, Cesar O Romero, Navita N Lopez, J Cameron Millar, Monica L Vetter, Alejandra Bosco

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Article in Experimental eye research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Joon SchwakopfDepartment of Neurobiology, University of Utah, Salt Lake City, UT, 84132, USA.
Cesar O RomeroDepartment of Neurobiology, University of Utah, Salt Lake City, UT, 84132, USA.
Navita N LopezDepartment of Neurobiology, University of Utah, Salt Lake City, UT, 84132, USA.
J Cameron MillarDepartment of Pharmacology and Neuroscience and North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.
Monica L VetterDepartment of Neurobiology, University of Utah, Salt Lake City, UT, 84132, USA.
Alejandra BoscoDepartment of Neurobiology, University of Utah, Salt Lake City, UT, 84132, USA. Electronic address: alejandra.bosco@neuro.utah.edu.

Funding

Schlemm’s canal targeted-Tie2 knockdown as a mouse model of adult-onset ocular hypertension and glaucomaR21EY033131 · NEI · UNIVERSITY OF UTAH · PI BOSCO, ALEJANDRA · 2021 to 2022
$414k
NEI NIH HHS R21 EY033131
6 · The paper itself

Abstract

Deficient Angiopoietin-Tie2 signaling is linked to ocular hypertension in glaucoma. Receptor Tie2/TEK expression and signaling at Schlemm's canal (SC) is indispensable for canal integrity and homeostatic regulation of aqueous humor outflow (AHO) and intraocular pressure (IOP), as validated by conditional deletion of Tie2, its ligands (Angpt1, Angpt2 and Angpt3/4) or regulators (Tie1 and PTPRB/VE-PTP). However, these Tie2/TEK knockouts and conditional knockouts are global or endothelial, preventing separation of systemic and ocular vascular defects that impact retinal or renal integrity. To develop a more targeted model of ocular hypertension induced by selective knockdown of Tie2/TEK expressed in SC, we combined the use of viral vectors to target the canal, and two distinct gene-editing strategies to disrupt the Tie2 gene. Adeno-associated virus (AAV2) is known to transduce rodent SC when delivered into the anterior chamber by intracameral injection. First, delivery of Cre recombinase via AAV2.Cre into R26

Indexed as

Disease Models, AnimalIntraocular PressureOcular HypertensionReceptor, TIE-2AnimalsAqueous HumorDependovirusGene Knockdown TechniquesGenetic VectorsLimbus CorneaeMiceMice, Inbred C57BLMice, KnockoutSchlemm's CanalTrabecular MeshworkReceptor, TIE-2Tek protein, mouseAAV2CRISPR/SaCas9GlaucomaIntraocular pressureSchlemm's canalTie2/TEKTrabecular meshwork

Identifiers

PMID39368692
PMCPMC11533709

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.