Evidence map›Paper›PMID 39368531›Full record

ArticleCryobiology2024

Kidney tissue loading reduces the critical cooling and warming rates of VS55 and VMP cryoprotective solutions.

Zonghu Han, Lakshya Gangwar, Bat-Erdene Namsrai, Joseph Sushil Rao, Cameron Scheithauer, Michael L Etheridge, Erik B Finger, John C Bischof, Jeunghwan Choi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cryobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Functional recovery of the adult murine hippocampus after cryopreservation by vitrification.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zonghu HanDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Lakshya GangwarDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Bat-Erdene NamsraiDepartment of Surgery, University of Minnesota, Minneapolis, MN, USA.
Joseph Sushil RaoDepartment of Surgery, University of Minnesota, Minneapolis, MN, USA; Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA.
Cameron ScheithauerDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Michael L EtheridgeDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Erik B FingerDepartment of Surgery, University of Minnesota, Minneapolis, MN, USA.
John C BischofDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA; Institute for Engineering in Medicine, University of Minnesota, Minneapolis, MN, USA. Electronic address: bischof@umn.edu.
Jeunghwan ChoiDepartment of Engineering Technologies, Safety, and Construction, Central Washington University, WA, USA. Electronic address: jeunghwan.choi@cwu.edu.

Funding

Organ banking for transplant—kidney cryopreservation by vitrification and novel nanowarming technologyR01DK117425 · NIDDK · UNIVERSITY OF MINNESOTA · PI JOHN C BISCHOF, Erik Brian Finger · 2018 to 2026
$4.9M
Cryopreservation and nanowarming enables whole liver banking for transplantation, cell therapy and biomedical researchR01DK132211 · NIDDK · UNIVERSITY OF MINNESOTA · PI JOHN C BISCHOF, Erik Brian Finger · 2023 to 2026
$2.6M
Breakthrough Tissue and Organ Preservation and Transplantation Using Scaled-Up Nanowarming TechnologyR01HL135046 · NHLBI · UNIVERSITY OF MINNESOTA · PI BISCHOF, JOHN C, FINGER, ERIK BRIAN · 2017 to 2020
$2.6M
NHLBI NIH HHS R01 HL135046NIDDK NIH HHS R01 DK117425NIDDK NIH HHS R01 DK132211
6 · The paper itself

Abstract

Critical cooling and warming rates (CCR and CWR) are two important calorimetric properties of cryoprotective agents (CPA) solutions, and achieving these rates is generally regarded as the critical criterion for successful vitrification and rewarming. In 1996, Peyridieu et al. discovered that the measured critical rates are reduced inside kidney tissue equilibrated with 30 % (w/w) 2,3-butanediol compared to its free CPA solution. In general, they found a ∼5-fold reduction for CCR and a >100-fold reduction for CWR. However, to our knowledge, no follow-up studies have been conducted. We revisit this important concept, understanding that tissues never fully equilibrate with full-strength 100 % CPAs during perfusion. We therefore performed measurements in a range of dilutions of two commonly employed CPA cocktails, including 75-100 % VS55 (41.25-55.00 % w/v) and 90-100 % VMP (48.60-54.00 % w/v) equilibrated with kidney tissues vs. free solution. The measured reduction in the kidney was up to 5-fold for CCR and 9-fold for CWR. After discussing possible mechanisms for this effect, curves that fit the dilution to the observed reduction in critical rate were constructed to allow extrapolation for differentially loaded tissues, which can guide the follow-up studies to find the more concentrated CPA (>8.4 M VMP) in the M22 family to achieve human-sized kidney vitrification and rewarming.

Indexed as

CryopreservationCryoprotective AgentsKidneyVitrificationAnimalsCold TemperatureMaleRatsCryoprotective AgentsCCRCWRKidney tissuesOrgan vitrificationVMPVS55

Identifiers

PMID39368531
PMCPMC11611618

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.