Evidence map›Paper›PMID 39367051›Full record

ArticleScientific reports2024

Comparison of human pluripotent stem cell differentiation protocols to generate neuroblastoma tumors.

Bo Cheng, Wanqi Fang, Steven Pastor, Alexander R March, Tania Porras, Hong-Wei Wu, Miriam Velez, Chintan Parekh, John M Maris, Shahab Asgharzadeh and 1 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bo ChengCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Wanqi FangCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Steven PastorDivision of Oncology and Center for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Alexander R MarchCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Tania PorrasCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Hong-Wei WuCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Miriam VelezCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Chintan ParekhCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
John M MarisDivision of Oncology and Center for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Shahab AsgharzadehCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA.
Miller HuangCancer and Blood Disease Institutes, Children's Hospital Los Angeles, The Saban Research Institute, 4650 Sunset Blvd #57, Los Angeles, CA, 90027, USA. mhuang@chla.usc.edu.

Funding

USC/NORRIS COMPREHENSIVE CANCER CENTER (CORE) SUPPORTP30CA014089 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Fumito Ito · 1985 to 2026
$181.4M
Discovering mechanisms of neuroblastoma tumorigenesis to improve patient outcomesR35CA220500 · NCI · CHILDREN'S HOSP OF PHILADELPHIA · PI JOHN M MARIS · 2017 to 2026
$8.7M
Identification of novel cooperating partners of MYCN in neuroblastoma tumorigenesisR00CA197484 · NCI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI HUANG, MILLER · 2019 to 2021
$747k
NCI NIH HHS P30 CA014089NCI NIH HHS R00 CA197484NCI NIH HHS R35 CA220500NIH HHS R00CA197484NIH HHS R35 CA220500
6 · The paper itself

Abstract

Neuroblastoma is the most common pediatric extracranial solid tumor and is derived from trunk neural crest cells (tNCC) and its progenitor sympathoadrenal (SA) cells. While human pluripotent stem cell (PSC) models of neuroblastoma have been described, the PSC were differentiated using protocols that made neural crest cells, but not specifically the trunk subtype. Here, we compared four recent protocols to differentiate pluripotent stem cells (PSC) toward SA cells and examined their efficiency at generating SA cells along with earlier cell states (neuromesodermal progenitors [NMP], tNCC), as well as generating MYCN-driven tumors. Interestingly, the protocols that created cells with the highest level of NMP markers did not produce cells with the highest tNCC or SA cell markers. We identified a protocol that consistently produced cells with the highest level of SA markers using two PSC lines of different genders. This protocol also generated tumors with the highest level of PHOX2B, a marker of neuroblastoma. Transcriptionally, however, each protocol generates tumors that resemble neuroblastoma. Two of the protocols repeatedly produced adrenergic neuroblastoma whereas the other two protocols were ambiguous. Thus, we identified a protocol that reliably generates adrenergic neuroblastoma.

Indexed as

Cell DifferentiationNeural CrestNeuroblastomaPluripotent Stem CellsAnimalsFemaleHomeodomain ProteinsHumansMaleMiceN-Myc Proto-Oncogene ProteinTranscription FactorsHomeodomain ProteinsMYCN protein, humanNBPhox proteinN-Myc Proto-Oncogene ProteinTranscription FactorsHuman pluripotent stem cellsMYCNNeuroblastomaSympathoadrenal cell

Identifiers

PMID39367051
PMCPMC11452544

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.