Evidence map›Paper›PMID 39366204›Full record

ArticleBiomaterials advances2025

Development of silk microfiber-reinforced bioink for muscle tissue engineering and in situ printing by a handheld 3D printer.

Meenakshi Kamaraj, Omid Rezayof, Alison Barer, Hansoul Kim, Nafiseh Moghimi, Akshat Joshi, Mehmet R Dokmeci, Ali Khademhosseini, Farshid Alambeigi, Johnson V John

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Journal of tissue engineering
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Meenakshi KamarajTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Omid RezayofWalker Department of Mechanical Engineering and Texas Robotics, The University of Texas at Austin, TX, USA.
Alison BarerTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Hansoul KimWalker Department of Mechanical Engineering and Texas Robotics, The University of Texas at Austin, TX, USA.
Nafiseh MoghimiTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Akshat JoshiTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Mehmet R DokmeciTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Ali KhademhosseiniTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Farshid AlambeigiWalker Department of Mechanical Engineering and Texas Robotics, The University of Texas at Austin, TX, USA.
Johnson V JohnTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA. Electronic address: jjohn@terasaki.org.

Funding

A Novel Semi-autonomous Surgeon-in-the-loop in situ Robotic Bioprinting System for Functional and Cosmetic Restoration of Volumetric Muscle Loss InjuriesDP2AR082471 · NIAMS · UNIVERSITY OF TEXAS AT AUSTIN · PI ALAMBEIGI, FARSHID · 2022 to 2025
$2.3M
Engineering the open porous nanofibrous microsphere integrated fibrillar hydrogel for the co-delivery of antibacterial and angiogenic agents aimed at the rapid diabetic wound repairR01DK134903 · NIDDK · TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION · PI Johnson Vitharikunnil John · 2023 to 2026
$2.2M
NIAMS NIH HHS DP2 AR082471NIDDK NIH HHS R01 DK134903
6 · The paper itself

Abstract

Volumetric muscle loss (VML) presents a significant challenge in tissue engineering due to the irreparable nature of extensive muscle injuries. In this study, we propose a novel approach for VML treatment using a bioink composed of silk microfiber-reinforced silk fibroin (SF) hydrogel. The engineered scaffolds are predesigned to provide structural support and fiber alignment to promote tissue regeneration in situ. We also validated our custom-made handheld 3D printer performance and showcased its potential applications for in situ printing using robotics. The fiber contents of SF and gelatin ink were varied from 1 to 5 %. Silk fibroin microfibers reinforced ink offered increased viscosity of the gel, which enhanced the shape fidelity and mechanical strength of the bulk scaffold. The fiber-reinforced bioink also demonstrated better cell-biomaterial interaction upon printing. The handheld 3D printer enabled the precise and on-demand fabrication of scaffolds directly at the defect site, for personalized and minimally invasive treatment. This innovative approach holds promise for addressing the challenges associated with VML treatment and advancing the field of regenerative medicine.

Indexed as

FibroinsPrinting, Three-DimensionalTissue EngineeringTissue ScaffoldsAnimalsBiocompatible MaterialsBioprintingGelatinHumansHydrogelsInkMusclesMuscle, SkeletalSilkBiocompatible MaterialsFibroinsGelatinHydrogelsSilkfiber reinforcementHandheld 3D printerSilk fibroinTissue regenerationVolumetric muscle loss

Identifiers

PMID39366204
PMCPMC11560616

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.