Evidence map›Paper›PMID 39366119›Full record

ReviewCurrent opinion in structural biology2024

Recent advances in correlative cryo-light and electron microscopy.

Joshua A Pierson, Jie E Yang, Elizabeth R Wright

Abstract readReview
In one paragraph

Review in Current opinion in structural biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
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  10. Cloneable contrast across all biological length scales.Journal of structural biology · 2026
    Article
  11. Article
  12. A structural perspective on α-glucan catabolism in oxygenic phototrophs.The Plant journal : for cell and molecular biology · 2026
    Review
  13. Article
  14. Article
  15. Review
  16. Cryogenic light microscopy of vitrified samples with angstrom precision.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Joshua A PiersonDepartment of Biochemistry, University of Wisconsin, Madison, WI, USA.
Jie E YangDepartment of Biochemistry, University of Wisconsin, Madison, WI, USA; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
Elizabeth R WrightDepartment of Biochemistry, University of Wisconsin, Madison, WI, USA; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Morgridge Institute for Research, Madison, WI, USA. Electronic address: erwright2@wisc.edu.

Funding

The Midwest Center for Cryo-Electron TomographyU24GM139168 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WRIGHT, ELIZABETH R · 2020 to 2025
$41.7M
Structural Biology of Alpha-Synuclein in Lewy Body DementiaRF1NS110436 · NINDS · WASHINGTON UNIVERSITY · PI KOTZBAUER, PAUL T, RIENSTRA, CHAD M · 2023 to 2023
$4.2M
Structural Investigations Of Macromolecular Complexes Critical To hRSV Life CycleR01GM114561 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SANTANGELO, PHILIP J, WRIGHT, ELIZABETH R · 2016 to 2019
$1.6M
Structure and Function of Prokaryotic Appendages by Cryo-Electron TomographyR01GM104540 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WRIGHT, ELIZABETH R · 2013 to 2017
$1.4M
Role of Vpu, Tetherin, and Siglec-1 in HIV-1 ReplicationR01GM132068 · NIGMS · CINCINNATI CHILDRENS HOSP MED CTR · PI SPEARMAN, PAUL W. · 2018 to 2018
$424k
NIGMS NIH HHS R01 GM104540NIGMS NIH HHS R01 GM114561NIGMS NIH HHS R01 GM132068NIGMS NIH HHS U24 GM139168NINDS NIH HHS RF1 NS110436
6 · The paper itself

Abstract

Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo-EM) to produce contextually relevant high-resolution structural snapshots of biological systems. Innovations in sample preparation, instrumentation, imaging, and data processing have advanced the field of cryo-EM. This review focuses on prior work and recent developments in the field of cryo- EM that support further integration of technologies for correlative microscopy workflows.

Indexed as

Cryoelectron MicroscopyAnimalsHumansImage Processing, Computer-AssistedMicroscopy, Fluorescence

Identifiers

PMID39366119
PMCPMC11602379

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.