Evidence map›Paper›PMID 39365816›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2024

Comprehensive stable-isotope tracing of glucose and amino acids identifies metabolic by-products and their sources in CHO cell culture.

Jacqueline E Gonzalez, Harnish Mukesh Naik, Eleanor H Oates, Venkata Gayatri Dhara, Brian O McConnell, Swetha Kumar, Michael J Betenbaugh, Maciek R Antoniewicz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jacqueline E Gonzalez *Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.
Harnish Mukesh Naik *Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.ORCID 0000-0002-8303-4219
Eleanor H OatesDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0002-5382-6557
Venkata Gayatri DharaDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.
Brian O McConnellDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.
Swetha KumarDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.
Michael J BetenbaughDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.
Maciek R AntoniewiczDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0002-0087-2875

Funding

NSF (NSF) 1624684
6 · The paper itself

Abstract

Mammalian cell culture processes are widely utilized for biotherapeutics production, disease diagnostics, and biosensors, and hence, should be optimized to support robust cell growth and viability. However, toxic by-products accumulate in cultures due to inefficiencies in metabolic activities and nutrient utilization. In this study, we applied comprehensive

Indexed as

Amino AcidsCarbon IsotopesCricetulusGlucoseAnimalsCell Culture TechniquesCHO CellsCricetinaeGas Chromatography-Mass SpectrometryImmunoglobulin GIsotope LabelingAmino AcidsCarbon IsotopesGlucoseImmunoglobulin Gamino acid catabolismmammalian cell culturemass spectrometrymetabolic by-productsstable isotope labeling

Identifiers

PMID39365816
PMCPMC11474065

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.