Evidence map›Paper›PMID 39365062›Full record

ArticlemBio2024

A dual program for CRP-mediated regulation in bacterial alarmone (p)ppGpp.

Li Zhao, Shi-Yu Zhou, Yu Fu, Jin-Long Shen, Bin-Cheng Yin, Di You, Bang-Ce Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. A systems-level atlas of carbon-response transcriptional states inProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Review
  4. Review
  5. Prokaryotic homeostasis - a solution to thrive and survive.Frontiers in molecular biosciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Li ZhaoLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Shi-Yu ZhouLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yu FuLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Jin-Long ShenLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Bin-Cheng YinLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Di YouLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID 0000-0001-6445-4798
Bang-Ce YeLab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID 0000-0002-5555-5359

Funding

MOE | Fundamental Research Funds for the Central Universities (Fundamental Research Fund for the Central Universities) JKF01221617MOE | Fundamental Research Funds for the Central Universities (Fundamental Research Fund for the Central Universities) JKF01231816MOST | National Key Research and Development Program of China (NKPs) 2020YFA0909100MOST | National Natural Science Foundation of China (NSFC) 32070066Shanghai Pilot Program for Basic Research 22TQ1400100-14
6 · The paper itself

Abstract

Gene expression and proper downstream cellular functions upon facing environmental shifts depend on the combined and cooperative regulation of genetic networks. Here, we identified cAMP receptor protein (CRP) as a master regulator of (p)ppGpp (guanosine tetra- and penta-phosphate) homeostasis. Via CRP-mediated direct transcriptional regulation of the (p)ppGpp synthetase/hydrolase RelA and SpoT, cAMP-CRP stimulates pervasive accumulation of (p)ppGpp under glucose-limiting conditions. Notably, CRP exerts a nonclassical property as a translational regulator through YfiQ-dependent acetylation of ribosome protein S1 at K247, which further enhances the translation of RelA, SpoT, and CRP itself. From a synthetic biology perspective, this self-activating feedback loop for (p)ppGpp synthesis highlights the function of

Indexed as

Cyclic AMP Receptor ProteinEscherichia coliGene Expression Regulation, BacterialGuanosine PentaphosphateAcetylationEscherichia coli ProteinsGTP PyrophosphokinaseGuanosine TetraphosphateLigasesPyrophosphatasesRibosomal ProteinsTranscription Factor RelAcrp protein, E coliCyclic AMP Receptor ProteinEscherichia coli ProteinsGTP Pyrophosphokinaseguanosine 3',5'-polyphosphate synthetasesGuanosine PentaphosphateGuanosine TetraphosphateLigasesPyrophosphatasesrelA protein, E coliRibosomal Proteinsribosomal protein S1Transcription Factor RelAcAMP-CRP(p)ppGppprotein acetylationtranscriptional regulationtranslational regulation

Identifiers

PMID39365062
PMCPMC11559003

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.