Evidence map›Paper›PMID 39363333›Full record

ArticleBMC research notes2024

Serological and molecular survey of Toxoplasma Gondii in aborted livestock fetuses from Northeast Iran.

Reza Shafiei, Nima Firouzeh, Mohammad Taghi Rahimi

Abstract read
In one paragraph

Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Reza ShafieiVector-Borne Disease Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Nima FirouzehVector-Borne Disease Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran. Nimafirouzeh4@gmail.com.ORCID http://orcid.org/0000-0003-3314-7271
Mohammad Taghi RahimiDepartment of Basic Medical Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Funding

North Khorasan, University of Medical Sciences (NKUMS) IR.NKUMS.REC.1400.117
6 · The paper itself

Abstract

backgroundToxoplasmosis not only leads to abortion in humans but also in herbivores, which causes significant financial and quality-adjusted life-year losses. The present study aimed to determine the prevalence of toxoplasmosis in aborted fetuses via serological and molecular assays. Moreover, the genotypes of the obtained isolates were detected.

methodsSerological and molecular methods were used to study aborted fetuses from Bojnourd City, North Khorasan Province, Iran, which included 52 ovines and 16 bovines. Nested PCR of the B1 gene was used to detect parasite DNA in brain tissues. The PCR-RFLP method for the GRA6 gene was used to determine the genotype of T. gondii.

resultsOut of 68 aborted fetuses, 16.1% showed the presence of anti-T. gondii IgG. Among these, 11.7% were identified in bovine fetuses and 4.4% in ovine fetuses. Additionally, two (2.94%) samples of ovine tested positive for anti-T. gondii IgM. Our PCR analysis detected parasite DNA in two cases (2.94%) among 11 IgG-positive samples. All obtained isolates belong to type I of T. gondii.

conclusionInfection with Type I of T. gondii during the neonatal period may partly be responsible for abortion and economic losses in livestock farming in our studied region. To understand the molecular epidemiology and genotypes of T. gondii associated with abortion, further evaluation of aborted samples from different geographical locations is necessary.

Indexed as

Aborted FetusToxoplasmaToxoplasmosis, AnimalAbortion, VeterinaryAnimalsAntibodies, ProtozoanCattleCattle DiseasesDNA, ProtozoanFemaleGenotypeImmunoglobulin GImmunoglobulin MIranLivestockPolymerase Chain ReactionAntibodies, ProtozoanDNA, ProtozoanImmunoglobulin GImmunoglobulin MAbortionGenotypingMolecularRuminant fetusesToxoplasma

Identifiers

PMID39363333
PMCPMC11451051

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.