ArticleMethods in molecular biology (Clifton, N.J.)2025
Standardized Golden Gate Assembly Metadata Representation Using SBOL.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows.ACS synthetic biology · 2026Article
- High-throughput yeast engineering in biofoundries: towards autonomous and scalable synthetic biology.FEMS yeast research · 2026Review
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Authors and funding
3 authors.
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Abstract
Synthetic biology, also known as engineering biology, is an interdisciplinary field that applies engineering principles to biological systems. One way to engineer biological systems is by modifying their DNA. A common workflow involves creating new DNA parts through synthesis and then using them in combination with other parts through assembly. Assembly standards such as MoClo, Phytobricks, and Loop are based on Golden Gate, and provide a framework for combining parts. The Synthetic Biology Open Language (SBOL) has implemented a best practice for representing build plans to communicate them to other practitioners through whiteboard designs and in a machine-readable format for communication with lab automation tools. Here we present a software tool for creating SBOL representations of build plans to simulate type IIS-mediated assembly reactions and store relevant metadata.
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Registered trials
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