Evidence map›Paper›PMID 39361215›Full record

ArticleAntonie van Leeuwenhoek2024

Involvement of adhesins (EcpD, FdeC, FimH) expressed in mammary pathogenic Escherichia coli on adhesion to bovine mammary epithelial cells.

Yusaku Tsugami, Taketoshi Iwata, Aoi Sugiyama, Megumi Onishi, Kei-Ichi Nakajima, Makoto Osaki, Yuya Nagasawa

Abstract read
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In one paragraph

Article in Antonie van Leeuwenhoek, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yusaku TsugamiNational Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan.ORCID http://orcid.org/0000-0001-7175-7981
Taketoshi IwataNational Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
Aoi SugiyamaNational Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan.
Megumi OnishiNational Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan.
Kei-Ichi NakajimaDepartment of Biochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka Higashi, Asahikawa City, Hokkaido, 078-8510, Japan.
Makoto OsakiNational Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
Yuya NagasawaNational Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan. ynagasawa@affrc.go.jp.ORCID http://orcid.org/0000-0003-1392-6454

Funding

F-REI JPFR23020108F-REI JPFR24020108Kuribayashi Scholarship and Academic Foundation R6-6Research project for technologies to strengthen the international competitiveness of Japan's agriculture and food industry 21452290
6 · The paper itself

Abstract

Mammary pathogenic Escherichia coli (MPEC) causes mastitis, which results in substantial economic losses to the dairy industry. A high percentage of Escherichia coli isolated from cows with clinical mastitis harbor adhesin genes, such as fimH. However, it is unclear whether these adhesins are important in the adhesion of MPEC to bovine mammary epithelial cells (BMECs). Therefore, we investigated the effect of adhesins (EcpD, FdeC, and FimH) in MPEC on adherence to the bovine mammary epithelium using cultured BMECs. For this purpose, we used wild-type MPEC as well as single- and double-mutants of fimH, ecpD, and fdeC, and performed adhesion assays with BMECs. First, BMECs were cultured in the presence of lactogenic hormones to induce milk component production and tight junction formation. The bacterial count of the wild-type strain that adhered to the BMECs increased in a dose-dependent manner. In deletion mutant strains, the ΔfimH strain showed lower adhesion (P < 0.05), whereas the adhesion ratio of the ΔecpD and ΔfdeC strains was not statistically different compared with that of the wild-type strain (P > 0.05). Additionally, the fimH/fdeC double-deletion mutants showed the lowest adhesion to BMECs. In conclusion, FimH is crucial in the adhesion of MPEC to BMECs. Overall, our work identifies FimH or FimH/FdeC as interesting targets for future drugs or vaccines to improve the treatment, prevention or chronicity of mastitis induced by MPEC.

Indexed as

Adhesins, Escherichia coliBacterial AdhesionEpithelial CellsEscherichia coliMammary Glands, AnimalAnimalsCattleEscherichia coli InfectionsEscherichia coli ProteinsFemaleFimbriae ProteinsMastitis, BovineAdhesins, Escherichia coliEscherichia coli ProteinsFimbriae ProteinsfimH protein, E coliAdhesionBovine mammary epithelial cellEscherichia coliMastitisMPEC

Identifiers

PMID39361215

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.