Evidence map›Paper›PMID 39361031›Full record

ArticleArchives of microbiology2024

Improving the activity of an inulosucrase by rational engineering for the efficient biosynthesis of low-molecular-weight inulin.

Dawei Ni, Zhaolin Huang, Shuqi Zhang, Yang Yang, Xiaoyong Liu, Wei Xu, Wenli Zhang, Wanmeng Mu

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Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dawei NiState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Zhaolin HuangState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Shuqi ZhangState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Yang YangDepartment of Food and Bioengineering, Beijing Vocational College of Agriculture, Beijing, 102442, China.
Xiaoyong LiuShandong Haizhibao Ocean Technology Co., Ltd, Weihai, 264333, Shandong, China.
Wei XuState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Wenli ZhangState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China. wenlizhang@jiangnan.edu.cn.
Wanmeng MuState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Funding

National Natural Science Foundation of China No. 22278183National Natural Science Foundation of China No. 22308118Natural Science Foundation of Jiangsu Province No. BK20231045Natural Science Foundation of Shandong Province No. ZR2023QC040the R&D Program of Beijing Municipal Education Commission No. KM202312448003
6 · The paper itself

Abstract

Inulin, a widely recognized prebiotic, has diverse applications across various industrial sectors. Although inulin is primarily produced through plant extraction, there is growing interest in enzymatic synthesis as an alternative. The enzymatic production of inulin from sucrose, which yields polymers with degrees of polymerization similar to those of plant-derived inulin, shows potential as a viable replacement for traditional extraction methods. In this study, an inulosucrase from Neobacillus bataviensis was identified, demonstrating a non-processive mechanism specifically tailored for synthesizing inulin with polymerization degrees ranging from 3 to approximately 40. The enzyme exhibited optimal activity at pH 6.5 and 55 °C, efficiently producing inulin with a yield of 50.6%. Ca

Indexed as

HexosyltransferasesInulinBacillalesBacterial ProteinsCalciumEnzyme StabilityHydrogen-Ion ConcentrationMolecular Dynamics SimulationMolecular WeightMutagenesis, Site-DirectedProtein EngineeringSucroseTemperatureBacterial ProteinsCalciumHexosyltransferasesInulininulosucraseSucroseInulosucraseLow-molecular-weight inulinMolecular dynamics simulationsNon-processive mechanismRational design

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.