ArticleeLife2024
Pectin methylesterase activity is required for RALF1 peptide signalling output.
Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Peptide hormones in shaping root system architecture and environmental adaptation: Current advances and translational perspectives.Plant communications · 2026Review
- Loss of galactan synthesis in pea (Pisum sativum) causes defects in organ expansion and is associated with increased extensin content.Plant molecular biology · 2026Article
- Pectin Structural Dynamics: Developmental and Evolutionary Perspectives.Plants (Basel, Switzerland) · 2026Review
- Pectin under stress: Pectin methylesterases at the core of cell wall remodeling, integrity sensing, and signaling in plants.Plant communications · 2026Review
- Endogenous RALF peptide function is required for powdery mildew host colonization.The New phytologist · 2026Article
- A symbiotic MLO gene regulates root development via RALF34-triggered Ca2+ signaling in Lotus japonicus.Plant physiology · 2026Article
- Biolayer Interferometry (BLI) to Quantify RALF1-Pectin Interactions.Bio-protocol · 2026Article
- Plant cell wall remodeling and peptide signaling under abiotic and biotic stress.Plant communications · 2026Review
- Insights into the roles of RALF peptides and CrRLK1L receptors in fruit ripening.The Plant journal : for cell and molecular biology · 2026Review
- Peptide signaling at the intersection of growth, nutrient sensing, and stress responses.The Plant journal : for cell and molecular biology · 2026Review
- Memory in the wall: expanding our understanding of the roles of plant cell walls.The New phytologist · 2026Review
- The Arabidopsis phosphatase PP2C12 negatively regulates LRX-RALF-FER-mediated cell wall integrity sensing.The EMBO journal · 2026Article
- The function of the Arabidopsis receptor kinase THESEUS1 in plant cell wall integrity maintenance: from evolutionary origin to future perspectives.The Plant journal : for cell and molecular biology · 2026Review
- PbrRALF5/10 prevents incompatible pollen tube death by reconstructing the methyl-esterified pectin and reactive oxygen species metabolism of pear in vitro.Plant molecular biology · 2025Article
- From roots to reproduction: the multifaceted roles of rapid alkalinization factor and epidermal patterning factor peptides in plants.Journal of experimental botany · 2025Review
- The interplay of RALF structural and signaling functions in plant-microbe interactions.PLoS pathogens · 2025Review
- Promiscuity and specificity in ligand sensing by plant cell surface receptors.PLoS pathogens · 2025Article
- Genome identification of the LRR-RLK gene family in maize (Zea mays) and expression analysis in response to Fusarium verticillioides infection.BMC plant biology · 2025Article
- FERONIA: A Malectin-Domain Receptor Kinase with Intricate Signaling Mechanisms and Profound Importance to Plant Wellness.The Yale journal of biology and medicine · 2025Review
- Article
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Authors and funding
8 authors.
Funding
Abstract
The extracellular matrix plays an integrative role in cellular responses in plants, but its contribution to the signalling of extracellular ligands largely remains to be explored. Rapid alkalinisation factors (RALFs) are extracellular peptide hormones that play pivotal roles in various physiological processes. Here, we address a crucial connection between the de-methylesterification machinery of the cell wall component pectin and RALF1 activity. Pectin is a polysaccharide, contributing to the structural integrity of the cell wall. Our data illustrate that the pharmacological and genetic interference with pectin methyl esterases (PMEs) abolishes RALF1-induced root growth repression. Our data suggest that positively charged RALF1 peptides bind negatively charged, de-methylesterified pectin with high avidity. We illustrate that the RALF1 association with de-methylesterified pectin is required for its FERONIA-dependent perception, contributing to the control of the extracellular matrix and the regulation of plasma membrane dynamics. Notably, this mode of action is independent of the FER-dependent extracellular matrix sensing mechanism provided by FER interaction with the leucine-rich repeat extensin (LRX) proteins. We propose that the methylation status of pectin acts as a contextualizing signalling scaffold for RALF peptides, linking extracellular matrix dynamics to peptide hormone-mediated responses.
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Registered trials
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