Evidence map›Paper›PMID 39358469›Full record

ArticleScientific reports2024

Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs.

Apidsada Chorpunkul, Usa Boonyuen, Kriengsak Limkittikul, Wachiraporn Saengseesom, Wallaya Phongphaew, Iyarath Putchong, Penpitcha Chankeeree, Sirin Theerawatanasirikul, Amin Hajitou, Surachet Benjathummarak and 3 more

Abstract read
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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Apidsada ChorpunkulDepartment of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Usa BoonyuenDepartment of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Kriengsak LimkittikulDepartment of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Wachiraporn SaengseesomQueen Saovabha Memorial Institute (WHO Collaborating Center for Research on Rabies), Thai Red Cross Society, Bangkok, 10330, Thailand.
Wallaya PhongphaewDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Iyarath PutchongDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Penpitcha ChankeereeDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Sirin TheerawatanasirikulDepartment of Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Amin HajitouCancer Phage Therapy Group, Department of Brain Sciences, Faculty of Medicine, Imperial College London, Hammersmith Hospital, London, W12 0NN, UK.
Surachet BenjathummarakCenter of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Pannamthip PitaksajjakulDepartment of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Porntippa LekcharoensukDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
Pongrama RamasootaDepartment of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand. pongrama.ram@mahidol.ac.th.

Funding

National Research Council of Thailand N42A670624National Research Council of Thailand PHD60I0075
6 · The paper itself

Abstract

Animal rabies is a potentially fatal infectious disease in mammals, especially dogs. Currently, the number of rabies cases in pet dogs is increasing in several regions of Thailand. However, no passive postexposure prophylaxis (PEP) has been developed to combat rabies infection in animals. As monoclonal antibodies (MAbs) are promising biological therapies for postinfection, we developed a canine-neutralizing MAb against rabies virus (RABV) via the single-chain variable fragment (scFv) platform. Immunized phage-displaying scFv libraries were constructed from PBMCs via the pComb3XSS system. Diverse canine VHVLκ and VHVLλ libraries containing 2.4 × 10

Indexed as

Antibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralRabiesRabies virusSingle-Chain AntibodiesAnimalsCell Surface Display TechniquesDog DiseasesDogsImmunizationMolecular Docking SimulationPeptide LibraryAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralPeptide LibrarySingle-Chain Antibodies

Identifiers

PMID39358469
PMCPMC11447112

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.