ArticleProceedings of the National Academy of Sciences of the United States of America2024
Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Thermo-Responsive Living Microspheroids Enable a Regenerative Living Disk-Drive System for DNA Data Storage.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.Nature communications · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Sustainable bioenergy manufacturing in plants.Plant communications · 2026Review
- Advances in luminescence-based tracing technologies: applications and future perspectives.Biodesign research · 2026Review
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Engineering glowing plants: recent progress and future directions for application-oriented design.Frontiers in bioengineering and biotechnology · 2026Review
- Article
- Establishment and maintenance of NRT2.1 inter-individual variability in plants.PLoS genetics · 2025Article
- Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications.JACS Au · 2025Review
- Engineered Bacteria for Disease Diagnosis and Treatment Using Synthetic Biology.Microbial biotechnology · 2025Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five-color auto-bioluminescence system named Nano-lanternX (NLX), which can be expressed in bacterial, mammalian, and plant hosts, thereby enabling auto-bioluminescence in various living organisms. Utilizing spectral unmixing, we achieved the successful observation of multicolor auto-bioluminescence, enabling detailed single-cell imaging across both bacterial and mammalian cells. We have also expanded the applications of the NLX system, such as multiplexed auto-bioluminescence imaging for gene expression, protein localization, and dynamics of biomolecules within living mammalian cells.
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Registered trials
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