Evidence map›Paper›PMID 39356423›Full record

ArticleActa parasitologica2024

Home-Made Lateral Flow Test Strip Versus POC-CCA Assay for Detection of Active Schistosomiasis in Egypt.

Manal Kamel, Faten Salah, Zeinab Demerdash, Sara Maher, Hanan El-Baz, Nahla Zakaria, Rania Abu-Shady, Amany Saad, Salwa Hassan, Doaa Abdel Aziz

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta parasitologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Manal KamelImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Faten SalahImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Zeinab DemerdashImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Sara MaherImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt. saram6756@gmail.com.
Hanan El-BazImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Nahla ZakariaClinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Rania Abu-ShadyClinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Amany SaadImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Salwa HassanImmunology Department, Theodor Bilharz Research Institute, Kornish El Nil Street, Giza, Egypt.
Doaa Abdel AzizClinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFor years, the Kato-Katz (KK) technique has been considered the gold standard for diagnosing schistosomiasis. The aim of this study was to compare the effectiveness of our previously developed gold nanoparticle-based lateral flow test strip (AuNPs-LFTS) for diagnosing active Schistosoma mansoni with that of the commercially available point-of-care Circulating Cathodic Antigen detection (POC-CCA) kit.

methodsIn this study, we collected sixty positive and twenty negative urine samples from patients in endemic hot spots in the Nile Delta, as well as from patients visiting the internal medicine clinic at Theodor Bilharz Research Institute (TBRI). We produced monoclonal antibodies (MAbs) against S. mansoni soluble egg antigen (SEA) from cloned hybridoma cells (4D/1D). These MAbs were conjugated with gold and mesoporous silica nanoparticles, and used to develop the LFTS.

resultsThe LFTS demonstrated a limit of detection (LoD) of 3 ng/ml. The sensitivity and specificity of the developed LFTS were found to be 96.7% and 95%, respectively, compared to 85% and 90% for the POC-CCA detection kit. The cases were divided into groups based on egg count in the stool, categorized as light, moderate, and heavy infections. The sensitivity of the LFTS in the group with light infection was higher than that of the POC-CCA. When using the KK technique (eggs per gram of stool sample [EPG]) as the reference test, the kappa value for the nano-based strips was 0.902, compared to 0.672 for the CCA strips, indicating an almost perfect agreement between KK and our developed LFTS.

conclusionThese results confirm the reliability and effectiveness of the LFTS compared to commercially available kits for rapid, sensitive, and early diagnosis of schistosomiasis. However, it is recommended to conduct further assessments of the developed strip on a larger scale with a broader range of cases before considering its introduction to local or international markets.

Indexed as

Antigens, HelminthGoldSchistosoma mansoniSchistosomiasis mansoniSensitivity and SpecificityAnimalsAntibodies, MonoclonalEgyptFecesHumansLimit of DetectionMetal NanoparticlesParasite Egg CountPoint-of-Care SystemsReagent StripsAntibodies, MonoclonalAntigens, HelminthGoldReagent StripsLateral flow testMonoclonal antibodiesNanoparticlesSchistosomiasis

Identifiers

PMID39356423
PMCPMC11649741

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.