Evidence map›Paper›PMID 39354309›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Reversed-Phase Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Method for High-Throughput Lipidomic Quantitation.

Zuzana Vaňková, Ondřej Peterka, Robert Jirásko, Michal Holčapek

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zuzana VaňkováFaculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
Ondřej PeterkaFaculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
Robert JiráskoFaculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
Michal HolčapekFaculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic. Michal.Holcapek@upce.cz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method is optimized for the quantitation of a large number of lipid species in biological samples, primarily in human plasma and serum. The method uses a C18 bridged ethylene hybrid (BEH) column (150 × 2.1 mm; 1.7 μm) for the separation of lipids from 23 subclasses with a total run time of 25 min. Lipid species separation allows the resolution of isobaric and isomeric lipid forms. A triple quadrupole mass spectrometer is used for targeted lipidomic analysis using multiple reaction monitoring (MRM) in the positive ion mode. Data are evaluated by Skyline software, and the concentrations of analytes are determined using internal standards per each individual lipid class.

Indexed as

Chromatography, Reverse-PhaseLipidomicsLipidsChromatography, High Pressure LiquidHigh-Throughput Screening AssaysHumansLiquid Chromatography-Mass SpectrometryMass SpectrometrySoftwareTandem Mass SpectrometryLipidsHigh-throughput lipidomicsMass spectrometryPlasmaQuantitationReversed-phaseSerumUltrahigh-performance liquid chromatography

Identifiers

PMID39354309

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.